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用于检测HIV gp41包膜蛋白的肽功能化生物传感器上的Python驱动阻抗分析

Python-driven impedance profiling on peptide-functionalized biosensor for detection of HIV gp41 envelope protein.

作者信息

Ezer Mehmet, Uygun Zihni Onur

机构信息

Department of Urology, Faculty of Medicine, Kafkas University, 36100 Kars, Turkey.

Department of Medical Biochemistry, Faculty of Medicine, Kafkas University, 36100 Kars, Turkey.

出版信息

3 Biotech. 2025 Jul;15(7):229. doi: 10.1007/s13205-025-04400-8. Epub 2025 Jun 30.

DOI:10.1007/s13205-025-04400-8
PMID:40599495
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12209138/
Abstract

UNLABELLED

This study presents the first label-free impedimetric biosensor for the detection of HIV envelope protein gp41 using antimicrobial peptides (AMPs) as biorecognition receptors. The biosensor interface was enhanced with thiolated polystyrene and gold nanospheres to ensure stable peptide immobilization and resist nonspecific adsorption. Electrochemical impedance spectroscopy (EIS) confirmed each step of the electrode modification, while surface morphology was validated via scanning electron microscopy. A Python-based deep learning algorithm was applied to impedance data for efficient curve fitting and regression modeling. The biosensor demonstrated high sensitivity, with a linear detection range of 5-600 pg/mL, a regression coefficient ( ) of 0.9946, a limit of detection (LOD) of 1.62 pg/mL, and a limit of quantification (LOQ) of 4.91 pg/mL. Chronoimpedimetric (CI) detection revealed that gp41 binding occurred within 350 s. The biosensor showed excellent reproducibility (CV % = 0.22%), good selectivity with less than 12% signal variation in spiked serum, and robust stability, maintaining functionality after extended storage. These results highlight the biosensor's potential as a rapid, sensitive, and reproducible diagnostic platform for early HIV detection.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13205-025-04400-8.

摘要

未标记

本研究展示了首个用于检测HIV包膜蛋白gp41的无标记阻抗生物传感器，该传感器使用抗菌肽（AMPs）作为生物识别受体。生物传感器界面通过硫醇化聚苯乙烯和金纳米球进行了增强，以确保肽的稳定固定并抵抗非特异性吸附。电化学阻抗谱（EIS）确认了电极修饰的每一步，同时通过扫描电子显微镜验证了表面形态。一种基于Python的深度学习算法被应用于阻抗数据，以进行高效的曲线拟合和回归建模。该生物传感器表现出高灵敏度，线性检测范围为5 - 600 pg/mL，回归系数（ ）为0.9946，检测限（LOD）为1.62 pg/mL，定量限（LOQ）为4.91 pg/mL。计时阻抗检测（CI）表明gp41结合在350秒内发生。该生物传感器具有出色的重现性（CV% = 0.22%），在加标血清中具有良好的选择性，信号变化小于12%，并且具有强大的稳定性，在长时间储存后仍保持功能。这些结果突出了该生物传感器作为早期HIV检测的快速、灵敏且可重现的诊断平台的潜力。

补充信息

在线版本包含可在10.1007/s13205-025-04400-8获取的补充材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/353b/12209138/2cee2d26d066/13205_2025_4400_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/353b/12209138/41107d7e69e9/13205_2025_4400_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/353b/12209138/e4ebcc9542e2/13205_2025_4400_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/353b/12209138/0ab883207199/13205_2025_4400_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/353b/12209138/2cee2d26d066/13205_2025_4400_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/353b/12209138/41107d7e69e9/13205_2025_4400_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/353b/12209138/dd3e4db1f4fd/13205_2025_4400_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/353b/12209138/5b8dc704ee84/13205_2025_4400_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/353b/12209138/e5b38b8dba01/13205_2025_4400_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/353b/12209138/e4ebcc9542e2/13205_2025_4400_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/353b/12209138/0ab883207199/13205_2025_4400_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/353b/12209138/2cee2d26d066/13205_2025_4400_Fig7_HTML.jpg

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