Live bacteria found in gastric cancer tumor tissue.

BACKGROUND

Most research on gastric cancer and microorganisms has focused on as a causative agent and on the relationship between the microbiota and gastric cancer. There is no direct evidence of bacteria in gastric cancer tissues or their relationship with gastric cancer.

METHODS

Gastric cancer tissue samples were collected, and the bacteria within tumor tissues were observed by transmission electron microscopy. The bacteria cultured were stained by Gram Staining and then subjected to High-throughput third-generation full-length 16S rRNA amplicon sequencing. Pure cultures were used to infect AGS gastric cancer cells, followed by protein extraction and Western blotting to detect POF1B protein expression.

RESULTS

The bacteria were observed in tumor tissues through transmission electron microscopy and had been cultivated under microaerophilic and anaerobic conditions, specifically, then were identified as (), , and . The vacuolation and mortality of AGS cell infected with increased ( < 0.05) and the POF1B protein expression upregulated ( < 0.05).

CONCLUSION

The live bacteria, including , , and , have been found in gastric cancer tumor tissue. specifically upregulate the oncogenic protein POF1B expression. The pathway through which this bacterium enters tumor tissue and its role in the tumor microenvironment require further investigation.