Lv Xiaoqian, Song Pengxia, Zhang Nianhua, Guo Pengbo, Zhang Rujian, Wang Haitao, Hong Longyan, Xu Rui, Wang Yu, Lv Gang, Zhao Yinghui
Department of Clinical Laboratory Medicine, The first Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Laboratory of Metabolism and Gastrointestinal Tumor, Shandong, China.
Department of Pathogenic Biology, School of Clinical and Basic Medicine, Shandong First Medical University, Jinan, Shandong, China.
Front Microbiol. 2025 Jun 18;16:1591735. doi: 10.3389/fmicb.2025.1591735. eCollection 2025.
Most research on gastric cancer and microorganisms has focused on as a causative agent and on the relationship between the microbiota and gastric cancer. There is no direct evidence of bacteria in gastric cancer tissues or their relationship with gastric cancer.
Gastric cancer tissue samples were collected, and the bacteria within tumor tissues were observed by transmission electron microscopy. The bacteria cultured were stained by Gram Staining and then subjected to High-throughput third-generation full-length 16S rRNA amplicon sequencing. Pure cultures were used to infect AGS gastric cancer cells, followed by protein extraction and Western blotting to detect POF1B protein expression.
The bacteria were observed in tumor tissues through transmission electron microscopy and had been cultivated under microaerophilic and anaerobic conditions, specifically, then were identified as (), , and . The vacuolation and mortality of AGS cell infected with increased ( < 0.05) and the POF1B protein expression upregulated ( < 0.05).
The live bacteria, including , , and , have been found in gastric cancer tumor tissue. specifically upregulate the oncogenic protein POF1B expression. The pathway through which this bacterium enters tumor tissue and its role in the tumor microenvironment require further investigation.
大多数关于胃癌与微生物的研究都集中在幽门螺杆菌作为致病因子以及微生物群与胃癌的关系上。目前尚无胃癌组织中细菌的直接证据或其与胃癌的关系。
收集胃癌组织样本，通过透射电子显微镜观察肿瘤组织内的细菌。对培养的细菌进行革兰氏染色，然后进行高通量第三代全长16S rRNA扩增子测序。用纯培养物感染AGS胃癌细胞，随后进行蛋白质提取和蛋白质印迹法检测POF1B蛋白表达。
通过透射电子显微镜在肿瘤组织中观察到细菌，并在微需氧和厌氧条件下进行培养，具体鉴定为大肠杆菌、粪肠球菌和鹑鸡肠球菌。感染大肠杆菌的AGS细胞空泡化和死亡率增加（P<0.05），POF1B蛋白表达上调（P<0.05）。
在胃癌肿瘤组织中发现了包括大肠杆菌、粪肠球菌和鹑鸡肠球菌在内的活细菌。大肠杆菌特异性上调致癌蛋白POF1B的表达。这种细菌进入肿瘤组织的途径及其在肿瘤微环境中的作用需要进一步研究。
