Li Yiqing, Ma Yonghong, Li Guanyu, Jia Yanqing, Sun Chengxin, Zhou Liushaoqiu, Lei Wenqin, Zhang Tao
Department of Laboratory Medicine Affiliated Hospital of Zunyi Medical University Zunyi China.
School of Laboratory Medicine Zunyi Medical University Zunyi China.
Food Sci Nutr. 2025 Jul 1;13(7):e70562. doi: 10.1002/fsn3.70562. eCollection 2025 Jul.
has been utilized as a dietary supplement in health foods and for medicinal purposes, enjoying a long-standing acceptance throughout history. A neutral polysaccharide fraction (WPA-N) and three acidic polysaccharide fractions (WPA-1, WPA-2, and WPA-3) prepared from by water extraction and DEAE-cellulose were tested for their chemical structures and inhibitory effects on galectin-1-mediated bioactivity. The results showed that WPA-N was composed of Glc and Gal residues, while WPA-1, WPA-2, and WPA-3 were mainly constituted by GalA, Gal, Ara, Rha, as well as some other monosaccharide residues, with the molecular weights ranging from 68.7 kDa to 168.2 kDa. Galectin-1 has been identified as a mediator in the multi-step process of tumor cell aggregation, migration, and invasion, as well as in tumor-induced angiogenesis, including in cervical cancer. Except for WPA-N, three acidic fractions could inhibit galectin-1-mediated hemagglutination and also could inhibit the fluorescence intensity of galectin-1 protein, and WPA-3 showed the strongest activity. Besides, anti-cancer experiments showed three acidic fractions could inhibit the growth and migration of cervical cancer ME180 cells, and WPA-3 inhibited the proliferation and migration activity of ME180 better than others. Biolayer interferometry analysis found that WPA-3 had a very strong binding ability to galectin-1 protein, with a value of 26.8 nM. WPA-3 contained rhamnogalacturonan-I mainchain with branched galactan, arabinan, and arabinogalactans-II side chains and some homogalacturonan domain. These findings indicated the potential applications in functional food fields of polysaccharides.
它已被用作保健食品中的膳食补充剂和药用，在历史上一直被广泛接受。通过水提取和DEAE-纤维素从[具体物质]制备的中性多糖组分（WPA-N）和三种酸性多糖组分（WPA-1、WPA-2和WPA-3），对其化学结构和对半乳糖凝集素-1介导的生物活性的抑制作用进行了测试。结果表明，WPA-N由葡萄糖（Glc）和半乳糖（Gal）残基组成，而WPA-1、WPA-2和WPA-3主要由半乳糖醛酸（GalA）、半乳糖、阿拉伯糖（Ara）、鼠李糖（Rha）以及其他一些单糖残基构成，分子量范围为68.7 kDa至168.2 kDa。半乳糖凝集素-1已被确定为肿瘤细胞聚集、迁移和侵袭以及肿瘤诱导血管生成（包括宫颈癌）多步骤过程中的介质。除WPA-N外，三种酸性组分可抑制半乳糖凝集素-1介导的血凝反应，还可抑制半乳糖凝集素-1蛋白的荧光强度，且WPA-3活性最强。此外，抗癌实验表明，三种酸性组分可抑制宫颈癌ME180细胞的生长和迁移，且WPA-3比其他组分更能有效抑制ME180的增殖和迁移活性。生物层干涉分析发现，WPA-3与半乳糖凝集素-1蛋白具有非常强的结合能力，解离常数（KD）值为26.8 nM。WPA-3含有带有分支半乳聚糖、阿拉伯聚糖和阿拉伯半乳聚糖-II侧链以及一些同型半乳糖醛酸结构域的鼠李糖半乳糖醛酸聚糖-I主链。这些发现表明了[具体物质]多糖在功能性食品领域的潜在应用。
