从猪和人类中分离出的丙型流感病毒的蛋白质和核酸分析。

Protein and nucleic acid analyses of influenza C viruses isolated from pigs and man.

作者信息

Elliott R M, Yuanji G, Desselberger U

出版信息

Vaccine. 1985 Sep;3(3 Suppl):182-8. doi: 10.1016/0264-410x(85)90100-8.

Abstract

The virus-coded proteins and the genomes of influenza C virus isolates obtained from Chinese pigs in 1981-1982 and of human influenza C virus strains isolated between 1947 and 1981 were compared. Using SDS polyacrylamide gel electrophoresis and one-dimensional peptide mapping we found the virus-coded proteins of the pig influenza C viruses to be similar to those of human influenza C virus strains. The sizes of the genomes of human and pig influenza C viruses were indistinguishable. Genome analysis by oligonucleotide (ON) mapping revealed that the genomes of the pig influenza C viruses were very similar to but not identical with those of human influenza C virus strains. ON changes were found scattered over the whole genome. ON mapping of isolated segments of several influenza C virus strains suggested that two pig strains (C/P/B/10/81 and C/P/B/32/81) are related by a reassortment event which is likely to have occurred in nature. The rate of genome variation in influenza C viruses seemed to be similar to that seen in influenza B, and slower than that recorded for influenza A viruses.

摘要

对1981 - 1982年从中国猪身上分离得到的丙型流感病毒毒株的病毒编码蛋白和基因组，以及1947年至1981年间分离得到的人类丙型流感病毒毒株进行了比较。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳和一维肽图分析，我们发现猪丙型流感病毒的病毒编码蛋白与人类丙型流感病毒毒株的相似。人类和猪丙型流感病毒基因组的大小难以区分。通过寡核苷酸（ON）图谱进行的基因组分析表明，猪丙型流感病毒的基因组与人类丙型流感病毒毒株的基因组非常相似，但并不相同。在整个基因组中都发现了寡核苷酸变化。对几种丙型流感病毒毒株的分离片段进行的寡核苷酸图谱分析表明，两个猪毒株（C/P/B/10/81和C/P/B/32/81）通过一次重配事件相关联，这一事件很可能发生在自然环境中。丙型流感病毒的基因组变异率似乎与乙型流感病毒的相似，且比甲型流感病毒记录的变异率要慢。