Ahmadzadeh Mozhgan, Shahamiri Kamal, Raeisi Mohammad, Jafari Negar, Kamian Shaghayegh, Ejlalidiz Mahsa, Kalaki Niloufar Sadat
Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Cancer Rep (Hoboken). 2025 Jul;8(7):e70232. doi: 10.1002/cnr2.70232.
Colorectal cancer (CRC) is a prevalent form of cancer globally and ranks as the second most common cause of cancer-related deaths. Long non-coding RNAs (lncRNAs) are regulatory RNAs that influence gene expression. EVADR and LUESCC are two novel lncRNAs specifically expressed in tumors of glandular origin, such as the colon.
This study aimed to investigate the expression of EVADR and LUESCC in colorectal tumor tissues and evaluate their potential as diagnostic and prognostic biomarkers in CRC.
Fifty cases of colorectal tumor tissues, formalin-fixed, paraffin-embedded (FFPE) from individuals with sporadic CRC, referred from the Pathology Department of Imam Hossein Hospital in Tehran, Iran, were analyzed. The expression patterns of LUESCC and EVADR lncRNAs in CRC patients were examined.
The study reveals upregulation of LUESCC and EVADR lncRNAs in colorectal cancer (CRC) patients compared to normal tissues, with fold changes of 3.52 (p < 0.001) for LUESCC and 3.08 (p < 0.001) for EVADR. ROC curve analysis indicates an area under the curve (AUC) of 0.75 for LUESCC and 0.86 for EVADR, suggesting strong diagnostic potential. Additionally, differential expression analysis shows correlations between lncRNA levels and tumor differentiation grades.
This study highlights the potential of LUESCC and EVADR lncRNAs as biomarkers for CRC diagnosis and prognosis. However, limitations include a small sample size that may affect the generalizability of the findings and a lack of functional assays to elucidate their roles in tumor biology. Further research is needed to validate these findings and explore the underlying mechanisms.
结直肠癌（CRC）是全球常见的癌症形式，是癌症相关死亡的第二大常见原因。长链非编码RNA（lncRNAs）是影响基因表达的调控RNA。EVADR和LUESCC是两种在腺源性肿瘤（如结肠）中特异性表达的新型lncRNAs。
本研究旨在调查EVADR和LUESCC在结直肠肿瘤组织中的表达，并评估它们作为CRC诊断和预后生物标志物的潜力。
分析了从伊朗德黑兰伊玛目侯赛因医院病理科转诊的50例散发性CRC患者的福尔马林固定、石蜡包埋（FFPE）结直肠肿瘤组织。检测了CRC患者中LUESCC和EVADR lncRNAs的表达模式。
研究显示，与正常组织相比，CRC患者中LUESCC和EVADR lncRNAs上调，LUESCC的倍数变化为3.52（p < 0.001），EVADR为3.08（p < 0.001）。ROC曲线分析表明，LUESCC的曲线下面积（AUC）为0.75，EVADR为0.86，表明具有很强的诊断潜力。此外，差异表达分析显示lncRNA水平与肿瘤分化程度之间存在相关性。
本研究强调了LUESCC和EVADR lncRNAs作为CRC诊断和预后生物标志物的潜力。然而，局限性包括样本量小可能影响研究结果的普遍性，以及缺乏功能分析来阐明它们在肿瘤生物学中的作用。需要进一步研究来验证这些发现并探索潜在机制。
