Fischer A B, Buchet J P, Lauwerys R R
Arch Toxicol. 1985 Aug;57(3):168-72. doi: 10.1007/BF00290882.
The cytotoxicity of trivalent and pentavalent inorganic arsenic salts was determined in mouse fibroblasts in vitro. Concentrations of As (III) in the microM range led to a reduction of proliferation and viability with a concomitant increase in LDH release and stimulation of lactic acid production. Similar effects were noted with approximately 10-fold greater molar concentrations of As(V). Cells pretreated with a low As(III) concentration are less sensitive to toxic doses of As(III) or As(V). Uptake of As(III) by the fibroblasts is greater than that of As(V). Both forms of inorganic arsenic are converted intracellularly to monomethylarsonic (MMA) and dimethylarsinic (DMA) acids, which are then released into the culture medium. In As-pretreated cells, which are more resistant to As toxicity, biotransformation of inorganic arsenic to MMA and DMA is increased.
在体外对小鼠成纤维细胞测定了三价和五价无机砷盐的细胞毒性。微摩尔范围内的As(III)浓度导致细胞增殖和活力降低，同时乳酸脱氢酶（LDH）释放增加以及乳酸生成受到刺激。在大约摩尔浓度高10倍的As(V)情况下也观察到类似效应。用低浓度As(III)预处理的细胞对As(III)或As(V)的毒性剂量敏感性较低。成纤维细胞对As(III)的摄取大于对As(V)的摄取。两种形式的无机砷在细胞内均转化为一甲基胂酸（MMA）和二甲基胂酸（DMA），然后释放到培养基中。在对砷毒性更具抗性的经砷预处理的细胞中，无机砷向MMA和DMA的生物转化增加。
