Pakroo Shadi, Soltani Samira, Tarrah Armin, LaPointe Gisèle
Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph, Guelph, ON, Canada.
Gut Microbiome (Camb). 2025 Jun 13;6:e9. doi: 10.1017/gmb.2025.10007. eCollection 2025.
The Simulator of the Human Intestinal Microbial Ecosystem (SHIME) system was provided with baby feed for one week to stabilise the microbial community, followed by a 10-day period with baby feed and another 10-day period with adult feed. The study was conducted using sterilised and standardised feed formulations, which model dietary conditions in vitro. Following the transition from baby to adult feed, a significant reduction in the proportion of butyrate in comparison to total SCFA was found after transitioning to adult feed in both the transverse colon and distal colon bioreactors. Our findings suggest that abrupt early-life dietary changes from simple to complex carbohydrates as well as the exclusion of bovine milk proteins can transiently lower the ability of the microbiota to produce butyrate. The lack of additional microbial input leads to a delay or impairment of the adaptation to the modified feed composition. However, given the short treatment duration and sterilised feed composition, these findings should be interpreted within the limitations of this in vitro model. A reduction in butyrate concentration following the transition to adult feed may reflect a temporary shift in microbial metabolic activity rather than a long-term impact on energy extraction efficiency in vivo.
人类肠道微生物生态系统模拟器（SHIME）系统先用婴儿配方奶喂养一周以稳定微生物群落，随后是10天的婴儿配方奶喂养期和另外10天的成人配方奶喂养期。该研究使用经过灭菌和标准化的饲料配方进行，这些配方模拟了体外饮食条件。从婴儿配方奶过渡到成人配方奶后，在横结肠和远端结肠生物反应器中，与总短链脂肪酸相比，丁酸盐比例显著降低。我们的研究结果表明，生命早期从简单碳水化合物到复杂碳水化合物的突然饮食变化以及牛乳蛋白的排除会暂时降低微生物群产生丁酸盐的能力。缺乏额外的微生物输入会导致对改良饲料成分的适应延迟或受损。然而，鉴于治疗持续时间短和饲料成分经过灭菌处理，这些发现应在这个体外模型的局限性内进行解释。过渡到成人配方奶后丁酸盐浓度的降低可能反映了微生物代谢活性的暂时转变，而不是对体内能量提取效率的长期影响。
