Brady L J, Brady P S, Romsos D R, Hoppel C L
Biochem J. 1985 Oct 15;231(2):439-44. doi: 10.1042/bj2310439.
Hepatic mitochondrial and peroxisomal oxidative capacities were studied in young (4-5 weeks old) and adult (6-9 months old) lean and obese ob/ob mice that were fed or starved for 24 or 48 h. The adult obese mice showed elevated capacity for mitochondrial oxidation (ng-atoms of O consumed/min per mg of protein) of lipid and non-lipid substrates, with the exception of pyruvate + malate, and elevated activities of citrate synthase and total carnitine palmitoyltransferase. Oxidative rates and enzyme activities were not affected by starvation of lean or obese mice, and both males and females responded similarly. Peroxisomal palmitoyl-CoA oxidation (nmol/min per mg of peroxisomal protein) was also increased in livers of adult obese mice and did not change with starvation. In young mice, hepatic mitochondrial and peroxisomal oxidative capacities in lean and obese mice were comparable. The increased mitochondrial and peroxisomal oxidative capacities appear to develop with maturation in obese ob/ob mice.
对年轻（4 - 5周龄）和成年（6 - 9月龄）的瘦型和肥胖型ob/ob小鼠进行研究，这些小鼠分别喂食或禁食24或48小时，以研究肝脏线粒体和过氧化物酶体的氧化能力。成年肥胖小鼠除丙酮酸 + 苹果酸外，脂质和非脂质底物的线粒体氧化能力（每毫克蛋白质每分钟消耗的氧纳摩尔原子数）有所提高，柠檬酸合酶和总肉碱棕榈酰转移酶的活性也有所提高。瘦型或肥胖型小鼠禁食对氧化速率和酶活性没有影响，雄性和雌性的反应相似。成年肥胖小鼠肝脏中的过氧化物酶体棕榈酰辅酶A氧化（每毫克过氧化物酶体蛋白质每分钟纳摩尔数）也增加，且禁食后不变。在年轻小鼠中，瘦型和肥胖型小鼠的肝脏线粒体和过氧化物酶体氧化能力相当。肥胖型ob/ob小鼠线粒体和过氧化物酶体氧化能力的增加似乎随着成熟而发展。
