Xiang Dan, Li Shaoying, Zuo Jiayi, Mao Chenchen, Lin Yangxuan, Long Cong, Cai Pingping, Liu Weiwei, Lu Xiaorong, Xiao Miaofang, Xie Wangkai, Chen Chenbin, Mei Dianfeng, Lin Kezhi, Han Zheng, Shen Xian, Xue Xiangyang, Shen Shurong
Wenzhou Key Laboratory of Cancer-related Pathogens and Immunity, Zhejiang International Cooperation Base for Tumor-Associated Pathogen and Host Interaction, Institute of Molecular Virology and Immunology, Department of Microbiology and Immunology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.
Department of Medical Oncology, Wenzhou Central Hospital, Wenzhou, China.
mBio. 2025 Aug 13;16(8):e0055125. doi: 10.1128/mbio.00551-25. Epub 2025 Jul 9.
Infectious diseases caused by pathogenic microorganisms and tumors arising from uncontrolled cell proliferation can be intricately linked through the lens of viromics. This study aimed to delineate the landscape of viral infections in gastric cancer, focusing on the relationship between pathogenic microorganisms and tumorigenesis. By conducting transcriptomic sequencing of gastric cancer tissues, we identified gastric cancer-associated viruses from raw transcriptomic data. This methodology was validated using public databases and experimental evidence, ensuring the authenticity of viral detection. Single-cell data further identified specific cells harboring these viruses and elucidated the correlation between viral gene expression and the genesis and progression of gastric cancer. Common tumor viruses, such as human endogenous retrovirus (HERV), Epstein-Barr virus (EBV), and human cytomegalovirus (HCMV), along with unique DNA viruses and bacteriophages, were detected in gastric cancer. Additionally, these viruses have been detected in both tumor and immune cells, indicating a close association between viral gene expression and the development of gastric cancer. This study advances the technique of screening viruses from transcriptomes, charting the distribution of viruses within tumors, clarifying the interplay between viruses and cancer, and identifying previously unknown viruses related to the occurrence and progression of cancer.
In our study, we have carefully examined the viral landscape in gastric cancer, supported by a series of thorough experiments that verify its reliability. Our approach has not only confirmed the presence of viruses known to be associated with cancer development but also identified a range of additional viral entities, including human cytomegalovirus (HCMV) and various herpesviruses, along with numerous bacteriophages. The high incidence of these viruses within tumor samples suggests they could be considered as potential biomarkers for early cancer detection. This method enhances our understanding of the role viruses play in cancer, which may assist scientists and medical professionals in identifying viral presence in cancers and could offer new angles for cancer prevention and the development of related measures. By identifying specific viruses linked to different cancers, we aim to improve patient outcomes.
从病毒组学的角度来看，由致病微生物引起的传染病与细胞不受控制增殖所产生的肿瘤可能存在复杂的联系。本研究旨在描绘胃癌中病毒感染的全貌，重点关注致病微生物与肿瘤发生之间的关系。通过对胃癌组织进行转录组测序，我们从原始转录组数据中鉴定出与胃癌相关的病毒。该方法通过公共数据库和实验证据进行了验证，确保了病毒检测的真实性。单细胞数据进一步确定了携带这些病毒的特定细胞，并阐明了病毒基因表达与胃癌发生和进展之间的相关性。在胃癌中检测到了常见的肿瘤病毒，如人类内源性逆转录病毒（HERV）、爱泼斯坦-巴尔病毒（EBV）和人类巨细胞病毒（HCMV），以及独特的DNA病毒和噬菌体。此外，这些病毒在肿瘤细胞和免疫细胞中均有检测到，表明病毒基因表达与胃癌的发展密切相关。本研究推进了从转录组中筛选病毒的技术，绘制了病毒在肿瘤内的分布图谱，阐明了病毒与癌症之间的相互作用，并鉴定出与癌症发生和进展相关的先前未知病毒。
在我们的研究中，我们通过一系列全面的实验仔细研究了胃癌中的病毒情况，验证了其可靠性。我们的方法不仅证实了已知与癌症发展相关的病毒的存在，还鉴定出了一系列额外的病毒实体，包括人类巨细胞病毒（HCMV）和各种疱疹病毒，以及众多噬菌体。这些病毒在肿瘤样本中的高发生率表明它们可被视为早期癌症检测的潜在生物标志物。该方法增强了我们对病毒在癌症中作用的理解，这可能有助于科学家和医学专业人员识别癌症中的病毒存在，并可能为癌症预防和相关措施的制定提供新的视角。通过识别与不同癌症相关的特定病毒，我们旨在改善患者的治疗结果。
