Knizia Dounya, Anil Erdem, Salhi Yasser, Shi Haiying, El Baze Abdelhalim, Kassem My Abdelmajid, Lakhssassi Naoufal, Nguyen Henry T, Meksem Khalid
School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States.
Division of Plant Science and Technology, University of Missouri, Columbia, MO, United States.
Front Plant Sci. 2025 Jun 25;16:1606321. doi: 10.3389/fpls.2025.1606321. eCollection 2025.
Soybean seed quality is influenced by its soluble sugar composition, with high sucrose content being desirable for nutritional and industrial applications. In contrast, excessive raffinose and stachyose levels are considered undesirable due to their adverse effects on gastrointestinal function in humans and monogastric animals. Therefore, developing soybean mutant lines with elevated sucrose content and optimal raffinose and stachyose content is desirable. In this study, we characterized twelve sucrose synthase genes through a comprehensive phylogenetic tree analysis, synteny analysis, gene structure evaluation, and variations in conserved domains. Additionally, we conducted a TILLING by Sequencing approach to identify EMS mutations in the characterized Sucrose synthase genes. Numerous mutations have been identified in soybean sucrose synthases that resulted in high sucrose content, including the sucrose synthases mutants SL446 (R582W) and F1115 (G249E) on with a sucrose content of 9.5% and 9.1%, respectively. The obtained soybean mutants with enhanced sugar content can be useful in breeding programs to improve soybean nutritional quality without potential developmental trade-offs.
大豆种子质量受其可溶性糖组成的影响，高蔗糖含量对于营养和工业应用而言是理想的。相比之下，棉子糖和水苏糖水平过高被认为是不理想的，因为它们会对人类和单胃动物的胃肠功能产生不利影响。因此，培育蔗糖含量升高且棉子糖和水苏糖含量最佳的大豆突变系是很有必要的。在本研究中，我们通过全面的系统发育树分析、共线性分析、基因结构评估以及保守结构域的变异，对12个蔗糖合酶基因进行了表征。此外，我们采用测序法进行靶向诱导基因组局部突变（TILLING），以鉴定所表征的蔗糖合酶基因中的甲基磺酸乙酯（EMS）突变。在大豆蔗糖合酶中已鉴定出许多导致高蔗糖含量的突变，包括蔗糖合酶突变体SL446（R582W）和F1115（G249E），其蔗糖含量分别为9.5%和9.1%。所获得的糖含量提高的大豆突变体可用于育种计划，以改善大豆营养品质，而不会有潜在的发育权衡。
