Zhang Ran, Wang Rui, Miao Xing, Li Hai, Song Puqing, Li Yuan, Lin Longshan
Laboratory of Marine Biodiversity Research, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, P.R. China.
Sci Data. 2025 Jul 10;12(1):1187. doi: 10.1038/s41597-025-05385-y.
Eelpouts (Zoarcidae) are known for their bipolar distributions and distinctive biogeographic histories. However, limited genomic data have hindered our understanding of their adaptive evolution. In this study, we present a thoroughly annotated chromosome-level genome assembly of pale eelpout (Lycodes pallidus) generated through the integration of Illumina, PacBio circular consensus, and Hi-C sequencing techniques. The final assembly spans 753.4 Mb, with its high quality confirmed by a scaffold N50 of 28.6 Mb and a Benchmarking Universal Single-Copy Ortholog (BUSCO) completeness of 99.3%. In comparison to other eelpouts and related fishes, the L. pallidus genome is larger and exhibits greater repetitive element content, accounting for approximately 45% of its total length. We annotated 21,419 protein-coding genes, a significant proportion of which are involved in signal transduction mechanisms and transcription. These findings provide valuable genetic resources for elucidating the evolutionary mechanisms underlying polar fish adaptation.
绵鳚科鱼类以其两极分布和独特的生物地理历史而闻名。然而，有限的基因组数据阻碍了我们对其适应性进化的理解。在本研究中，我们展示了通过整合Illumina、PacBio环形一致序列和Hi-C测序技术生成的苍白绵鳚（Lycodes pallidus）的一个经过全面注释的染色体水平基因组组装。最终组装的基因组跨度为753.4 Mb，其高质量通过28.6 Mb的支架N50和99.3%的基准通用单拷贝直系同源基因（BUSCO）完整性得到证实。与其他绵鳚科鱼类和相关鱼类相比，苍白绵鳚的基因组更大，并且重复元件含量更高，约占其总长度的45%。我们注释了21419个蛋白质编码基因，其中很大一部分参与信号转导机制和转录。这些发现为阐明极地鱼类适应背后的进化机制提供了宝贵的遗传资源。
