Keshavarzian A, Price Y E, Peters A M, Lavender J P, Wright N A, Hodgson H J
Dig Dis Sci. 1985 Dec;30(12):1156-60. doi: 10.1007/BF01314050.
The validity of 111In granulocyte scanning and fecal excretion measurement, as a reflection of loss of cells into the gastrointestinal tract, was studied using an autoradiographic technique in 11 patients in whom 111In granulocyte scan and colonoscopy were carried out simultaneously. 111In granulocytes were injected 1.5-4 hr prior to colonoscopy, and intraluminal fluid, mucosal brushings, and colonic biopsies were collected during the colonoscopy. In two patients with no histological evidence of inflammatory bowel disease, and four patients with clinically and histologically inactive inflammatory bowel disease, no 111Indium was detected in fluid, brushing, or biopsies. In five patients with active disease, 85% of the 111In activity in colonic fluid was precipitated by low-speed centrifugation. Autoradiography confirmed that the label remained attached to whole granulocytes in colonic fluid and mucosal brushings. Studies on biopsies, at intervals up to 4 1/2 hr following labeled granulocyte injection, demonstrated labeled polymorphonuclear neutrophils (PMNs) on the inflamed epithelial surface, with occasional cells in crypt abscesses by 110 min. We conclude that the techniques of 111In granulocyte scanning and fecal counting in patients with IBD are specifically measuring cell loss; labeled PMNs are capable of migrating through the gastrointestinal mucosa, in active disease, within 2 hr of administration.
在11例同时进行铟 - 111（¹¹¹In）粒细胞扫描和结肠镜检查的患者中，使用放射自显影技术研究了¹¹¹In粒细胞扫描和粪便排泄测量作为反映细胞向胃肠道丢失情况的有效性。在结肠镜检查前1.5 - 4小时注射¹¹¹In粒细胞，在结肠镜检查期间收集肠腔内液体、黏膜刷取物和结肠活检组织。在2例无炎症性肠病组织学证据的患者以及4例临床和组织学上非活动期炎症性肠病患者的液体、刷取物或活检组织中未检测到¹¹¹铟。在5例活动期疾病患者中，结肠液中85%的¹¹¹In活性通过低速离心沉淀。放射自显影证实标记物仍附着在结肠液和黏膜刷取物中的完整粒细胞上。对活检组织的研究显示，在注射标记粒细胞后长达4.5小时的间隔时间内，在炎症上皮表面可见标记的多形核中性粒细胞（PMN），到110分钟时隐窝脓肿中有偶尔的细胞。我们得出结论，炎症性肠病患者的¹¹¹In粒细胞扫描和粪便计数技术专门用于测量细胞丢失；在活动期疾病中，标记的PMN在给药后2小时内能够穿过胃肠道黏膜。
