Cleveland Imogen, Beeby Andrew, Vieira Márcia, Pina Fernando, Branco Paula S, Nabais Paula, Melo Maria J
LAQV-REQUIMTE, Department of Conservation and Restoration (DCR), Nova School of Science and Technology, 2829-516 Caparica, Portugal.
Department of Chemistry, Durham University, Durham DH1 3LE, UK.
Molecules. 2025 Jul 4;30(13):2860. doi: 10.3390/molecules30132860.
Natural colorants, with their sustainable origins, offer a promising alternative for various applications. Advanced studies have unveiled the remarkable properties, resilience, and durability of these ancient dyes, which our ancestors developed through sustainable material processing. This serves as a testament to the potential of sustainable solutions in our field. As part of our research, we prepared three medieval temperas using gum arabic, parchment glue, and casein glue. These tempera were explicitly designed to protect the purples obtained from extracts. A comprehensive multi-analytical approach guides our research on natural colorants. Central to this approach is the use of molecular fluorescence by microspectrofluorimetry, a key tool in our study. By analyzing the emission and excitation spectra in the visible range, we can identify specific formulations. This method is further supported by fingerprinting techniques, including Fourier Transform Infrared Spectroscopy (FTIR) and High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD). These are further complemented by Fiber Optics Reflectance Spectroscopy (FORS) and colorimetry. Building on our understanding of orcein purples, we have extended our research to purples derived from extracts. Our findings reveal the unique properties of , which can be accurately distinguished from orcein purples, highlighting the distinctiveness of each.
天然色素因其可持续的来源，为各种应用提供了一个有前景的替代方案。深入研究揭示了这些古老染料的卓越特性、韧性和耐久性，它们是我们的祖先通过可持续材料加工开发出来的。这证明了我们领域中可持续解决方案的潜力。作为我们研究的一部分，我们使用阿拉伯树胶、羊皮纸胶和酪蛋白胶制备了三种中世纪蛋彩画颜料。这些蛋彩画颜料专门设计用于保护从提取物中获得的紫色。一种全面的多分析方法指导我们对天然色素的研究。这种方法的核心是使用显微分光荧光法进行分子荧光分析，这是我们研究中的关键工具。通过分析可见光范围内的发射光谱和激发光谱，我们可以识别特定的配方。该方法还得到指纹技术的支持，包括傅里叶变换红外光谱（FTIR）和带二极管阵列检测的高效液相色谱（HPLC-DAD）。这些技术进一步由光纤反射光谱（FORS）和比色法补充。基于我们对地衣红紫色的理解，我们将研究扩展到了从提取物中获得的紫色。我们的研究结果揭示了的独特性质，它可以与地衣红紫色准确区分开来，突出了每种紫色的独特性。
