Kurin Elena, Hajská Marianna, Kostovčíková Ema, Dokupilová Kamila, Mučaji Pavel, Nagy Milan, Novotný Branislav, Bittner Fialová Silvia
Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojárov 10, 83232 Bratislava, Slovakia.
4th Department of Surgery, Faculty of Medicine, University Hospital Bratislava, Comenius University, Ružinovská 6, 82606 Bratislava, Slovakia.
Int J Mol Sci. 2025 Jun 28;26(13):6257. doi: 10.3390/ijms26136257.
This study explores the antioxidant activity of green tea (, GT) and peppermint ( × , PM) infusions, individually and in combination, focusing on how preparation methods affect their efficacy. Antiradical and intracellular antioxidant activity was assessed using DPPH, ABTS, and DCF assays, alongside interaction analysis via combination index (CI) and dose reduction index (DRI). HPLC analysis determined the polyphenolic profiles of GT and PM. GT showed the strongest antioxidant activity, with the lowest IC values (4.81 µg/mL in DPPH, 2.70 µg/mL in ABTS, 3.71 µg/mL in DCF), indicating potent radical-scavenging potential. PM exhibited moderate antiradical capacity but similar intracellular activity (IC = 3.80 µg/mL). Co-maceration followed by lyophilization of GT:PM extracts led to nearly additive interactions (CI~1.0) and allowed significant dose reduction (DRI up to 4.44). In contrast, post-mixed extracts showed assay-dependent effects, including antagonism in intracellular ROS inhibition (CI = 1.83). Equimolar mixtures of model polyphenols: EGCG, quercetin, and rosmarinic acid demonstrated enhanced effects, with the strongest synergy in ternary mixtures (CI = 0.67-0.86), potentially achievable in GT:PM combinations. These findings highlight that extract preparation critically influences antioxidant efficacy, supporting co-maceration as a promising strategy for developing effective functional formulations based on plant extract combinations.
本研究探讨了绿茶（GT）和薄荷（PM）浸液单独及混合后的抗氧化活性，重点关注制备方法如何影响其功效。使用DPPH、ABTS和DCF试验评估了抗自由基和细胞内抗氧化活性，并通过组合指数（CI）和剂量降低指数（DRI）进行相互作用分析。HPLC分析确定了GT和PM的多酚谱。GT表现出最强的抗氧化活性，IC值最低（DPPH中为4.81μg/mL，ABTS中为2.70μg/mL，DCF中为3.71μg/mL），表明其具有强大的自由基清除潜力。PM表现出中等的抗自由基能力，但细胞内活性相似（IC = 3.80μg/mL）。GT:PM提取物共浸渍后冻干导致几乎相加的相互作用（CI~1.0），并允许显著降低剂量（DRI高达4.44）。相比之下，后混合提取物显示出与试验相关的效应，包括细胞内ROS抑制中的拮抗作用（CI = 1.83）。模型多酚（表没食子儿茶素没食子酸酯、槲皮素和迷迭香酸）的等摩尔混合物显示出增强的效果，三元混合物中协同作用最强（CI = 0.67 - 0.86），这在GT:PM组合中可能实现。这些发现突出表明提取物制备对抗氧化功效有至关重要的影响，支持共浸渍作为开发基于植物提取物组合的有效功能制剂的一种有前景的策略。
