人类c-fes/fps原癌基因的结构
The structure of the human c-fes/fps proto-oncogene.
作者信息
Roebroek A J, Schalken J A, Verbeek J S, Van den Ouweland A M, Onnekink C, Bloemers H P, Van de Ven W J
出版信息
EMBO J. 1985 Nov;4(11):2897-903. doi: 10.1002/j.1460-2075.1985.tb04020.x.
Abstract
We have determined the complete nucleotide sequence of a human DNA fragment of approximately 13 kbp, which was shown by Southern blot analysis to contain the entire v-fes/fps cellular homolog. The v-fes/fps homologous sequences were dispersed over 11 kbp in 18 interspersed segments which were flanked by splice junctions. Fusion of these segments created a DNA fragment in which coding regions similar to those observed in the viral oncogenes v-fes of the Gardner-Arnstein (GA) and Snyder-Theilen (ST) strains of feline sarcoma virus and v-fps found in Fujinami sarcoma virus could be identified. A potential initiation site in the first exon was found. About 200 nucleotides downstream of a translational stop codon in the v-fes/fps homologous region, a poly(A) addition signal was identified. The deduced amino acid sequence has a molecular weight of 93 390 dalton resembling NCP92, the recently described human c-fes/fps product. The topography of human c-fes/fps appeared to resemble that of chicken c-fps.
摘要
我们已经确定了一段约13kbp的人类DNA片段的完整核苷酸序列，Southern印迹分析表明该片段包含整个v-fes/fps细胞同源物。v-fes/fps同源序列分散在11kbp的18个散布片段中，这些片段两侧为剪接连接点。这些片段的融合产生了一个DNA片段，其中可以鉴定出与在猫肉瘤病毒的Gardner-Arnstein（GA）和Snyder-Theilen（ST）株的病毒癌基因v-fes以及在藤浪肉瘤病毒中发现的v-fps中观察到的编码区域相似的编码区域。在第一个外显子中发现了一个潜在的起始位点。在v-fes/fps同源区域的翻译终止密码子下游约200个核苷酸处，鉴定出一个聚腺苷酸添加信号。推导的氨基酸序列的分子量为93390道尔顿，类似于最近描述的人类c-fes/fps产物NCP92。人类c-fes/fps的拓扑结构似乎类似于鸡c-fps的拓扑结构。
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