Tang Jun, Yang Xin, Yang Ming, Wu Zhenfeng, He Yanan, Zhang Chen, Zhang Dingkun
State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.
National Key Laboratory for the Modernization of Classical and Famous Prescriptions of Chinese Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, PR China.
Int J Biol Macromol. 2025 Sep;321(Pt 4):145902. doi: 10.1016/j.ijbiomac.2025.145902. Epub 2025 Jul 10.
Wound healing is a complex physiological process, and the continuous inflammatory response significantly contributes to the challenges associated with effective wound healing. Consequently, extensive research has focused on the development of functional wound dressings suitable for complex wound repair, aimed at facilitating rapid wound repair. In this study, we prepared a novel nanofibers material composed of polylactic acid (PLA) and Aconiti Lateralis Radix Praeparata polysaccharide (FPS) using the coaxial electrospinning technique to enhance wound healing capabilities. The nanofibers were characterized by SEM, TEM, FTIR, and TGA-DTG. Permeability and expansion rate experiments indicated that the nanofibers possessed favorable permeability and hygroscopicity, while tensile property assessments revealed their excellent tensile property and flexibility. In vitro hemolysis and cell experiments demonstrated that the nanofibers exhibited good biocompatibility and high safety. Furthermore, the wound healing efficacy of the nanofibers material was evaluated utilizing a rat full-thickness skin defect wound model, which revealed that the FPS-PLA nanofibers effectively promoted wound healing, accelerated collagen deposition, and induced RAW264.7 macrophages polarization toward the M2 phenotype and down-regulate myeloperoxidase (MPO) at the wound site. The nanofibers demonstrated remarkable capabilities in promoting wound repair and anti-inflammatory, showing great potential in the field of wound dressings.
伤口愈合是一个复杂的生理过程，持续的炎症反应显著加剧了有效伤口愈合所面临的挑战。因此，大量研究聚焦于开发适用于复杂伤口修复的功能性伤口敷料，旨在促进伤口快速修复。在本研究中，我们采用同轴静电纺丝技术制备了一种由聚乳酸（PLA）和炮制附子多糖（FPS）组成的新型纳米纤维材料，以增强伤口愈合能力。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、傅里叶变换红外光谱仪（FTIR）和热重 - 微商热重联用仪（TGA - DTG）对纳米纤维进行了表征。渗透性和膨胀率实验表明，纳米纤维具有良好的渗透性和吸湿性，而拉伸性能评估显示其具有优异的拉伸性能和柔韧性。体外溶血和细胞实验表明，纳米纤维表现出良好的生物相容性和高安全性。此外，利用大鼠全层皮肤缺损伤口模型评估了纳米纤维材料的伤口愈合效果，结果表明FPS - PLA纳米纤维有效地促进了伤口愈合，加速了胶原蛋白沉积，并诱导RAW264.7巨噬细胞向M2表型极化，同时下调了伤口部位的髓过氧化物酶（MPO）。该纳米纤维在促进伤口修复和抗炎方面表现出显著能力，在伤口敷料领域具有巨大潜力。
