Saito Yoshiro, Shimizu Misaki, Sato Mitsuharu, Masuda An, Arisawa Kotoko, Taguchi Keiko, Toyama Takashi, Motoike Ikuko N, Kinoshita Kengo, Koshiba Seizo, Yamamoto Masayuki, Takamura Toshinari
Laboratory of Molecular Biology and Metabolism, Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8578, Japan.
Tohoku Medical Megabank Organization, Tohoku University, Sendai, 980-8573, Japan.
Sci Rep. 2025 Jul 13;15(1):25319. doi: 10.1038/s41598-025-10581-2.
Essential trace elements, such as iron (Fe) and selenium (Se), play physiological roles in our body, whereas environmental toxic metals, such as arsenic (As), cadmium (Cd), and mercury (Hg), are known to be associated with various disease risks. However, the relationship between elements, biochemical parameters, and lifestyle habits based on multi-elemental analysis in healthy individuals has not been fully verified. Multi-elemental analysis is useful for evaluating the change in the concentration of these elements and metals. In the present study using totally 100 µL plasma samples from the Tohoku Medical Megabank (TMM) community-based cohort study (total of 506 specimens), we conducted a multi-elemental analysis to evaluate 14 elements in generally healthy subjects. We further determined Se-transporter selenoprotein P levels using the originally developed ELISA method, since increases and decreases in selenoprotein P levels are associated with various disease risks. Multiple correlation analyses between the obtained measured values and several factors suggest that elements such as Fe, Se, and Hg, as well as selenoprotein P levels, are associated with gender differences. We also found that factors such as Fe, Se, As, Hg, hematocrit value, hemoglobin (Hb) content, and HbA1c are correlated with selenoprotein P levels. Furthermore, correlations between Fe levels and Hb content and between As/Hg and fish consumption were found. These findings demonstrate the suitability of multi-elemental analyses with limited plasma sample amounts, clearly show gender-differentiated elements, and establish a significant relationship between selenoprotein P and Fe metabolism.
铁（Fe）和硒（Se）等必需微量元素在我们体内发挥着生理作用，而砷（As）、镉（Cd）和汞（Hg）等环境有毒金属则已知与各种疾病风险相关。然而，基于健康个体多元素分析的元素、生化参数和生活方式习惯之间的关系尚未得到充分验证。多元素分析对于评估这些元素和金属浓度的变化很有用。在本研究中，我们使用来自东北医学大数据库（TMM）社区队列研究的总共100微升血浆样本（共506个标本），对一般健康受试者中的14种元素进行了多元素分析。我们还使用最初开发的酶联免疫吸附测定（ELISA）方法测定了硒转运蛋白硒蛋白P的水平，因为硒蛋白P水平的升高和降低与各种疾病风险相关。对获得的测量值与几个因素之间的多重相关性分析表明，铁、硒和汞等元素以及硒蛋白P水平与性别差异有关。我们还发现，铁、硒、砷、汞、血细胞比容值、血红蛋白（Hb）含量和糖化血红蛋白（HbA1c）等因素与硒蛋白P水平相关。此外，还发现了铁水平与血红蛋白含量之间以及砷/汞与鱼类消费量之间的相关性。这些发现证明了在有限血浆样本量下进行多元素分析的适用性，清楚地显示了性别差异元素，并建立了硒蛋白P与铁代谢之间的重要关系。
