血清G蛋白偶联受体4和双糖链蛋白聚糖水平与椎间盘退变严重程度的相关性分析

Correlation Analysis of Serum G-Protein-Coupled Receptor 4 and Biglycan Levels with the Severity of Intervertebral Disc Degeneration.

作者信息

Gao Bingjie, Cui Yizhi, Qin Yexiao, Qu Chuncheng, Zhao Jiaqi, Li Xiaoning

机构信息

Second Clinical Medical School, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, People's Republic of China.

Department of Acupuncture, The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, People's Republic of China.

出版信息

Orthop Res Rev. 2025 Jul 8;17:289-297. doi: 10.2147/ORR.S525337. eCollection 2025.

DOI:10.2147/ORR.S525337

PMID:40654563

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12255263/

Abstract

OBJECTIVE

To investigate the correlation between serum levels of G protein-coupled receptor 4 (GPR4) and Biglycan (BGN) with the severity of Intervertebral Disc Degeneration (IVDD).

METHODS

A total of 162 patients with IVDD treated at our hospital from August 2023 to August 2024 were included. The general information of patients was retrospectively collected. MRI was used to assess IVDD severity using the Pfirrmann grading system. Serum GPR4 and BGN levels were measured by enzyme-linked immunosorbent assay (ELISA). Multiple linear regression analysis was performed to identify risk factors for IVDD severity. 's and 's correlation analyses were used to evaluate the relationships between serum GPR4, BGN, and IVDD severity. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic value of serum GPR4 and BGN in IVDD.

RESULTS

Significant differences in age and the proportion of diabetic patients as well as serum GPR4 and BGN were found among different Pfirrmann grades (<0.05). Serum GPR4 levels increased but BGN levels decreased with higher Pfirrmann grades (<0.05). Multiple linear regression analysis showed that age and serum GPR4 and BGN levels were risk factors for IVDD severity (<0.05). The results of the correlation analysis showed that serum GPR4 and age were positively correlated with the severity of IVDD (=0.651, =0.488, <0.001), while BGN was negatively correlated with the severity of IVDD (=-0.591, <0.001). The results of correlation analysis showed a negative correlation between serum GPR4 and BGN (<0.05). ROC curve analysis revealed that the AUC values for the diagnosis of IVDD using serum GPR4 alone, BGN alone, and the combination of GPR4 and BGN were 0.918, 0.811, and 0.919, respectively (<0.05). Moreover, the combination of GPR4 and BGN demonstrated higher sensitivity and specificity compared to either marker alone.

CONCLUSIONS

Serum GPR4 and BGN levels are identified as effective diagnostic indicators for IVDD, with serum GPR4 positively correlated but BGN negatively correlated with the severity of IVDD.

摘要

目的

探讨血清G蛋白偶联受体4（GPR4）和双糖链蛋白聚糖（BGN）水平与椎间盘退变（IVDD）严重程度之间的相关性。

方法

纳入2023年8月至2024年8月在我院接受治疗的162例IVDD患者。回顾性收集患者的一般资料。采用Pfirrmann分级系统，通过磁共振成像（MRI）评估IVDD的严重程度。采用酶联免疫吸附测定（ELISA）法检测血清GPR4和BGN水平。进行多元线性回归分析，以确定IVDD严重程度的危险因素。采用Pearson相关分析和Spearman相关分析评估血清GPR4、BGN与IVDD严重程度之间的关系。采用受试者工作特征（ROC）曲线分析评估血清GPR4和BGN对IVDD的诊断价值。

结果

不同Pfirrmann分级之间在年龄、糖尿病患者比例以及血清GPR4和BGN方面存在显著差异（P<0.05）。随着Pfirrmann分级升高，血清GPR4水平升高而BGN水平降低（P<0.05）。多元线性回归分析显示，年龄以及血清GPR4和BGN水平是IVDD严重程度的危险因素（P<0.05）。相关性分析结果显示，血清GPR4和年龄与IVDD严重程度呈正相关（r=0.651，r=0.488，P<0.001），而BGN与IVDD严重程度呈负相关（r=-0.591，P<0.001）。Spearman相关性分析结果显示血清GPR4与BGN呈负相关（P<0.05）。ROC曲线分析显示，单独使用血清GPR4、单独使用BGN以及GPR4与BGN联合诊断IVDD的曲线下面积（AUC）值分别为0.918、0.811和0.919（P<0.05）。此外，与单独使用任一标志物相比，GPR4与BGN联合使用显示出更高的敏感性和特异性。