Modrek Aram S, Huynh Khoi, Do Catherine, Karp Jerome, Ding Yingwen, Zhang Ze-Yan, Ezhilarasan Ravesanker, Valor Belen, Cova Giulia, Skok Jane A, Sulman Erik P
Department of Radiation Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine of USC, Los Angeles, CA, 90033.
Department of Pathology, New York University Langone Health, New York, NY 10016, USA.
bioRxiv. 2025 May 10:2025.05.07.652656. doi: 10.1101/2025.05.07.652656.
CRISPR-Cas9 guide-RNA design tools can be used to identify guide-RNAs that target single human genomic loci. However, these approaches limit their effect to a single locus. Here, we generate a database of potential individual guide-RNAs that target multiple sites in the genome at once. All guide-RNAs in this database are curated with on- and off-target quantification and enrichment scores for genomic elements such as gene elements, regulatory elements, repetitive elements, and transcription factor motifs. This tool enables rationale mass targeting of genomic loci with a single guide for functional studies. We created a web-app for user-friendly guide-RNA selection (https://modreklab.shinyapps.io/guiderna/).
CRISPR-Cas9引导RNA设计工具可用于识别靶向单个人类基因组位点的引导RNA。然而，这些方法的作用仅限于单个位点。在此，我们生成了一个潜在的单个引导RNA数据库，这些引导RNA可同时靶向基因组中的多个位点。该数据库中的所有引导RNA都经过筛选，具有针对基因元件、调控元件、重复元件和转录因子基序等基因组元件的靶向和脱靶定量以及富集分数。该工具能够通过单个引导对基因组位点进行合理的大规模靶向，以用于功能研究。我们创建了一个网络应用程序，以便用户友好地选择引导RNA（https://modreklab.shinyapps.io/guiderna/）。
