Green M H, Bridges B A, Rogers A M, Horspool G, Muriel W J, Bridges J W, Fry J R
Mutat Res. 1977 Jul;48(3-4):287-93. doi: 10.1016/0027-5107(77)90171-3.
We describe the use of a simplified bacterial fluctuation test to detect induced mutation, either incorporating liver microsomal or whole liver cell preparations. We have evaluated both types of test using three agents. The fluctuation assay seems somewhat slower, simpler and more sensitive than a conventional plating test with microsomes. A whole cell preparation appears marginally more effective than a microsomal fraction for metabolic activation of 9,10-dimethyl-1,2-benzanthracene, but rather less effective for benz(a)pyrene and 2-acetamidofluorene. Ultimately the usefulness of activation by whole cells may depend upon whether the method can give a correlation with carcinogenicity that is more quantitative than microsome methods and better reflects organ and species specificity.
我们描述了一种简化的细菌波动试验用于检测诱导突变的方法，该试验可加入肝微粒体或全肝细胞制剂。我们使用三种试剂对这两种类型的试验进行了评估。波动试验似乎比传统的微粒体平板试验稍慢、更简单且更灵敏。对于9,10-二甲基-1,2-苯并蒽的代谢活化，全细胞制剂似乎比微粒体部分略有效，但对于苯并(a)芘和2-乙酰氨基芴则效果较差。最终，全细胞活化的实用性可能取决于该方法能否给出与致癌性的相关性，这种相关性比微粒体方法更具定量性，并且能更好地反映器官和物种特异性。
