Glazer V M, Savov V M, Abilev S K, Shesterin S I, Beĭm A M
Nauchnye Doki Vyss Shkoly Biol Nauki. 1984(5):85-9.
The study of mutagenic effect of 2-aminoantracene and benz(alpha)pyrene on Salmonella triphimurium TA 100 in the Ames test-system in the presence of postmitochondrial fractions S-9 from carp liver with 3-methylcholantrene induced by microsomal oxidation system has been carried out. The metabolic activity and cytochrome P450 contence in carp liver microsomes have been shown to concede considerably those in rats liver. But these characteristics are sufficient for the use of fraction S-9 from carp liver for the study of genotoxic effect of these xenobiotics in the Ames test-system. Several regimes of storage of S-9 preparations from carp liver have been compared. S-9 preparations frozen immediately after isolation preserve their metabolic activity with respect to 2-aminoantracene and benz(alpha)pyrene well.
在微粒体氧化系统诱导的3-甲基胆蒽存在下，利用鲤鱼肝脏的线粒体后上清液S-9，在Ames试验系统中开展了2-氨基蒽和苯并（α）芘对鼠伤寒沙门氏菌TA 100诱变作用的研究。结果表明，鲤鱼肝脏微粒体中的代谢活性和细胞色素P450含量明显低于大鼠肝脏中的。但这些特性足以使鲤鱼肝脏的S-9组分用于Ames试验系统中这些外源生物的遗传毒性效应研究。比较了鲤鱼肝脏S-9制剂的几种保存方式。分离后立即冷冻的S-9制剂对2-氨基蒽和苯并（α）芘的代谢活性保存良好。
