Construction of an electrochemical sensor based on N-COF@MWCNT-COOH/Tri-AgNPs for sensitive detection of tyrosine in biological fluids.

A sensitive electrochemical sensor was constructed for tyrosine detection by collaborating a highly conductivity, excellent electrocatalysis and large surface of nitrogen-rich porous covalent organic framework in situ grown on three-dimensional conductive carboxylated multi-walled carbon nanotubes network and catalytic activity of triangular silver nanosheets. The prepared electrochemical sensing platform exhibited satisfactory results for detection of tyrosine with a wide linear range of 0.6-280 μM and a low detection limit of 0.017 μM, which were significantly superior to those of most existing electrochemical methods. At the same time, the developed sensor was successfully applied to the detection of tyrosine in real samples including human serum and urine and displayed high recoveries, good stability, reproducibility, and anti-interference ability. This study not only provides a novel sensing platform for tyrosine monitoring but also offers an innovative design concept for leveraging multifunctional nanocomposites in biosensing applications.