Zhang Xue, Sun Shulan, Cheng Siqi, Dai Junze, Du Furong, Wang Jingrui, Wei Dan, Yan Yichao, Liu Yefu
Central Laboratory, Cancer Hospital of Dalian University of Technology, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning, China.
China Medical University, Shenyang, Shenyang, Liaoning, China.
Front Cell Infect Microbiol. 2025 Jun 30;15:1601688. doi: 10.3389/fcimb.2025.1601688. eCollection 2025.
Since the early 20 century, there has been extensive discussion on the intricate relationship between pathogenic infection and tumors. However, most studies on host-pathogen interactions are performed based on the culture, immortalized cell lines or animal experiments. A significant challenge lies in accurately establishing a coculture model between tumors and pathogens under the three-dimensional (3D) context. Recently, the hybrid model system that incorporates 3D tumor organoids and two-dimensional cell lines have been gradually used to analyze the intricate relationship between pathogens and tumors, and several coculture techniques for tumor organoids and pathogens have also been developed. Therefore, this study systematically reviewed the preparation and identification of tumor organoids, coculture techniques with pathogens, and their clinical applications, aiming to further understand and simulate the interaction mechanism between the hosts and pathogens.
自20世纪初以来，关于病原体感染与肿瘤之间的复杂关系已展开了广泛讨论。然而，大多数关于宿主-病原体相互作用的研究是基于培养、永生化细胞系或动物实验进行的。一个重大挑战在于在三维（3D）环境下准确建立肿瘤与病原体之间的共培养模型。最近，结合3D肿瘤类器官和二维细胞系的混合模型系统已逐渐用于分析病原体与肿瘤之间的复杂关系，并且还开发了几种肿瘤类器官与病原体的共培养技术。因此，本研究系统综述了肿瘤类器官的制备与鉴定、与病原体的共培养技术及其临床应用，旨在进一步了解和模拟宿主与病原体之间的相互作用机制。
