Jhun JooYeon, Lee Donghwan, Na Hyun Sik, Cho Keun-Hyung, Lee Seung Yoon, Lee Jeong Su, Lee Young Joon, Kim Seok Jung, Park Sung-Hwan, Cho Mi-La
Lab of Translational ImmunoMedicine (LaTIM), Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea.
Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea.
J Inflamm (Lond). 2025 Jul 15;22(1):27. doi: 10.1186/s12950-025-00453-x.
Osteoarthritis (OA), a chronic degenerative disorder, induces pain, joint inflammation, and destruction of the articular cartilage matrix. Curcumin and omega-3 have been used as dietary supplements for OA due to their anti-inflammatory and antioxidant properties. However, there is no evidence demonstrating a synergistic effect in OA. The current study aimed to investigate the therapeutic effects and underlying mechanism of a combination of curcumin and omega-3 in the treatment of OA.
Wistar rats were injected with monosodium iodoacetate to induce OA. Oral treatments of a vehicle, curcumin, curcumin and omega 3, or celecoxib were administered. Pain was analyzed according to the paw withdrawal latency, paw withdrawal threshold, and weight bearing ability. The joint was isolated from OA rats, and cartilage damage was evaluated using histomorphological techniques, the Mankin scoring system, and micro computed tomography analysis. Protein expression in the joint was examined using immunohistochemistry. The expression levels of catabolic markers were measured in curcumin and omega-3-treated OA chondrocytes.
The OA animal model revealed diminished pain and cartilage conservation in response to the combined treatment. mRNA levels of matrix metalloproteinase 1 (MMP1), MMP3, and MMP13 were reduced in interleukin-1 beta-simulated human OA chondrocytes. Additionally, mitochondrial markers, cytochrome c oxidase 4, and TOMM20, were increased by the combination treatment.
These findings suggest promising therapeutic outcomes for the combined treatment of curcumin and omega-3 in OA patients.
骨关节炎（OA）是一种慢性退行性疾病，会引发疼痛、关节炎症以及关节软骨基质的破坏。姜黄素和ω-3因其抗炎和抗氧化特性，已被用作OA的膳食补充剂。然而，尚无证据表明它们在OA中具有协同作用。本研究旨在探究姜黄素和ω-3联合治疗OA的疗效及潜在机制。
向Wistar大鼠注射碘乙酸钠以诱导OA。分别给予溶剂、姜黄素、姜黄素与ω-3联合用药或塞来昔布进行口服治疗。根据爪部缩腿潜伏期、爪部缩腿阈值和负重能力分析疼痛情况。从OA大鼠分离关节，使用组织形态学技术、曼金评分系统和微型计算机断层扫描分析评估软骨损伤。采用免疫组织化学法检测关节中的蛋白表达。测定姜黄素和ω-3处理的OA软骨细胞中分解代谢标志物的表达水平。
OA动物模型显示联合治疗可减轻疼痛并保护软骨。在白细胞介素-1β模拟的人OA软骨细胞中，基质金属蛋白酶1（MMP1）、MMP3和MMP13的mRNA水平降低。此外，联合治疗可增加线粒体标志物细胞色素c氧化酶4和TOMM20。
这些发现表明姜黄素和ω-3联合治疗OA患者有望取得良好的治疗效果。
