Kiss L I, Ganago A O, Garab G I
J Biochem Biophys Methods. 1985 Oct;11(4-5):213-25. doi: 10.1016/0165-022x(85)90003-x.
Pigmented vesicular membranes embedded in polyacrylamide gel exhibit linear dichroism when the gel sample is squeezed [Abdourakhmanov, I.A., Ganago, A.O., Erokhin, Yu.E., Solov'ev, A.A. and Chugunov, V.A. (1979) Biochim. Biophys. Acta 546, 183-186]. The orientation technique of gel-squeezing was modified to enhance polarization effects in membrane vesicles of spherical symmetry. Model calculations were carried out to provide a tool for the quantitative evaluation of the dichroism of squeezed gel samples. The orientation angles of the dipoles can be calculated with reasonable precision by measuring two quantities: (i) the macroscopic deformation parameter of the gel sample, and (ii) a parameter (e.g. the polarization ratio of the fluorescence emission) characterizing the orientation of the transition dipoles in the membranes embedded in the squeezed gel. The validity of the model was confirmed through a series of polarization measurements relating to the fluorescence of chlorophyll a in membranes of osmotically shocked chloroplasts, 'blebs'.
当挤压聚丙烯酰胺凝胶中的色素囊泡膜时，嵌入其中的色素囊泡膜会呈现线二色性[阿卜杜勒拉赫马诺夫，I.A.，加纳戈，A.O.，叶罗欣，Yu.E.，索洛维耶夫，A.A.和丘古诺夫，V.A.（1979年）《生物化学与生物物理学报》546卷，第183 - 186页]。对凝胶挤压的取向技术进行了改进，以增强具有球对称性的膜囊泡中的偏振效应。进行了模型计算，为定量评估挤压凝胶样品的二色性提供了一种工具。通过测量两个量，可以合理精确地计算偶极子的取向角：（i）凝胶样品的宏观变形参数，以及（ii）一个表征挤压凝胶中嵌入膜内跃迁偶极子取向的参数（例如荧光发射的偏振比）。通过一系列与渗透休克叶绿体“小泡”膜中叶绿素a荧光相关的偏振测量，证实了该模型的有效性。
