Kumar Sushil, Sharma Vikrant, Thakur Nistha, Singh Baljit
Department of Chemistry, Himachal Pradesh University, Shimla 171005, India.
Department of Chemistry, Himachal Pradesh University, Shimla 171005, India.
Int J Biol Macromol. 2025 Apr;301:140477. doi: 10.1016/j.ijbiomac.2025.140477. Epub 2025 Jan 29.
Recently, significant progress has been made in the development of natural polysaccharide-derived functional copolymers for advanced biomedical uses. Herein, the main objective of the present research work was to explore the potential of gum acacia (GA) and tragacanth gum (TG) for developing network hydrogels to use in drug delivery (DD) applications. The copolymers were prepared by grafting of 3-sulfopropylacrlate (SPA) onto gum (GA-TG). FE-SEM, AFM, XRD, XPS, FTIR, C NMR and DSC techniques were applied for their characterizations and structural analysis. The physicochemical, morphological and biomedical properties of hydrogels were investigated. The optimized polymer network exhibited a mesh size (ξ) of 13.95 mm and a cross-linking density (ρ) of 6.44 × 10 mol/cm. FE-SEM and AFM revealed heterogeneous morphology and rough topology of copolymer hydrogels. The XRD revealed the amorphous state of the copolymer. FTIR and C NMR confirmed the incorporation of poly(SPA) chains onto gums. Diffusion of meropenem drug occurred in a sustained manner with a non-Fickian diffusion mechanism. The release profile of the drug was best described by the First-order kinetic model. The results of polymer-blood interactions revealed their non-haemolytic & non-thrombogenic features. Copolymers exhibited antioxidant nature and illustrated 40.72 ± 2.08 % scavenging ability during DPPH assay. The hydrogel demonstrated a mucoadhesive nature and required 100 ± 10 mN forces to separate from mucous membrane. The meropenem impregnated hydrogel exhibited antibacterial activity against Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) bacteria, respectively. The results of various properties demonstrated the suitability of network hydrogels for DD uses.
最近，在用于先进生物医学用途的天然多糖衍生功能共聚物的开发方面取得了重大进展。在此，本研究工作的主要目标是探索阿拉伯胶（GA）和黄芪胶（TG）在开发用于药物递送（DD）应用的网络水凝胶方面的潜力。通过将3-磺丙基丙烯酸酯（SPA）接枝到胶（GA-TG）上制备共聚物。采用场发射扫描电子显微镜（FE-SEM）、原子力显微镜（AFM）、X射线衍射（XRD）、X射线光电子能谱（XPS）、傅里叶变换红外光谱（FTIR）、碳核磁共振（C NMR）和差示扫描量热法（DSC）技术对其进行表征和结构分析。研究了水凝胶的物理化学、形态和生物医学性质。优化后的聚合物网络的网孔尺寸（ξ）为13.95 毫米，交联密度（ρ）为6.44×10摩尔/立方厘米。FE-SEM和AFM揭示了共聚物水凝胶的非均相形态和粗糙拓扑结构。XRD显示共聚物为非晶态。FTIR和C NMR证实了聚（SPA）链结合到胶上。美罗培南药物以非菲克扩散机制持续扩散。药物释放曲线用一级动力学模型描述最佳。聚合物与血液相互作用的结果显示其具有非溶血和非血栓形成特性。共聚物具有抗氧化性质，在二苯基苦味酰基自由基（DPPH）测定中表现出40.72±2.08%的清除能力。水凝胶表现出粘膜粘附性质，从粘膜分离需要100±10毫牛顿的力。美罗培南浸渍水凝胶分别对大肠杆菌（E. coli）、铜绿假单胞菌（P. aeruginosa）和金黄色葡萄球菌（S. aureus）具有抗菌活性。各种性质的结果表明网络水凝胶适用于药物递送用途。
