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CRISPR-Cas技术在检测新型冠状病毒变异株中的批判性综述

A Critical Review of the CRISPR-Cas Technology in the Detection of SARS-CoV-2 Variants.

作者信息

Zhang Jie, Li Juezhuo, Zhou Jiawei, Zhong Jiaye, Xu Yue, Mao Xiaolei, Xu Minghui, Luo Shuyin, Yang Yi, Hu Ruiyao, Liu Dong-Ang, Chen Shiyu, Qiu Yuting, Chen Keyi, Yuan Jinghua, Zhang Xinling, Li Xiaoping

机构信息

Key Laboratory of Artificial Organs and Computational Medicine in Zhejiang Province, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China.

Wycombe Abbey School Hangzhou, Hangzhou, China.

出版信息

Can J Infect Dis Med Microbiol. 2025 Jul 9;2025:9107724. doi: 10.1155/cjid/9107724. eCollection 2025.

DOI:10.1155/cjid/9107724
PMID:40678258
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12267969/
Abstract

Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still going on, and as the epidemic situation continues, the genome of SARS-CoV-2 is also mutating and evolving, resulting in more and more SARS-CoV-2 mutant strains, which have brought serious pressure on the prevention and control of COVID-19. Given that the COVID-19 is still spreading, it is extremely important to rapidly identify SARS-CoV-2 variants by nucleic acid assays. Thus, developing highly sensitive and specific assays that are suitable for field testing, high-throughput, and automation, as well as other diagnostic applications for SARS-CoV-2 variants, is urgently needed. This paper reviews the research progress of novel CRISPR-based diagnostic methods for SARS-CoV-2 variants.

摘要

由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的2019冠状病毒病（COVID-19）仍在持续，随着疫情的持续，SARS-CoV-2的基因组也在发生突变和进化，导致越来越多的SARS-CoV-2突变株出现，这给COVID-19的防控带来了严重压力。鉴于COVID-19仍在传播，通过核酸检测快速鉴定SARS-CoV-2变体极其重要。因此，迫切需要开发适用于现场检测、高通量和自动化的高灵敏度和特异性检测方法，以及用于SARS-CoV-2变体的其他诊断应用。本文综述了基于新型CRISPR的SARS-CoV-2变体诊断方法的研究进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fac/12267969/a21baf958427/CJIDMM2025-9107724.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fac/12267969/b7598cedf9f8/CJIDMM2025-9107724.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fac/12267969/e13468553e08/CJIDMM2025-9107724.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fac/12267969/a21baf958427/CJIDMM2025-9107724.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fac/12267969/b7598cedf9f8/CJIDMM2025-9107724.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fac/12267969/6307d36030b9/CJIDMM2025-9107724.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fac/12267969/1e6ea2fab27a/CJIDMM2025-9107724.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fac/12267969/e13468553e08/CJIDMM2025-9107724.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fac/12267969/a21baf958427/CJIDMM2025-9107724.005.jpg

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