Banerjee Aparna, Fabi João Paulo, Ormeño Catherine, Banerjee Srijan, Flores-Castañón Nicolás, Valenzuela Francisca, Sarkar Shrabana, Galán Alexander, Samanta Dipayan, Saxena Priya, Sani Rajesh K, Rao Manik Prabhu Narsing, Cabrera-Barjas Gustavo
Functional Polysaccharides Research Group, Instituto de Ciencias Aplicadas, Facultad de Ingeniería, Universidad Autónoma de Chile, Sede Talca, Talca, Chile.
Department of Food Science and Experimental Nutrition, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil; Food Research Center (FoRC), CePID-FAPESP (Research, Innovation and Dissemination Centers, São Paulo Research Foundation), CEPIX, São Paulo, SP, Brazil.
Int J Biol Macromol. 2025 Sep;321(Pt 1):146114. doi: 10.1016/j.ijbiomac.2025.146114. Epub 2025 Jul 17.
Deception Island, Antarctica, harbors unique polyextreme conditions fostering versatile microorganisms with significant biotechnological potential. This is due to genomic adaptation to extreme conditions that lead to metabolic alterations, such as the production of unique exopolysaccharides. Bacillus licheniformis F2LB, isolated from Deception Island, Antarctica, was evaluated for its exopolysaccharide's structural and biotechnological potential (EPS). Genome analysis revealed EPS biosynthesis, UV resistance, and thermal adaptation genes. The EPS with a putative glucogalactomannan backbone, comprised mainly of a sugar molar ratio Man: Glu: Xyl: Fuc (1: 0.7: 0.4: 0.3) and other sugars in trace amounts. Spectroscopy identified key glycosidic linkages, and morphology showed a rough, nonporous surface. Functionally, EPS demonstrated robust thermal stability (T 255 °C), remarkable antioxidant activities (~100 % H₂O₂ scavenging; DPPH: 75.5 %), good emulsifying ability (90 % in olive oil), and water (238.7 %) and oil-holding capacities (384.7 %) exceeding commercial xanthan gum. These findings underline its potential as a sustainable food additive.
南极洲的欺骗岛拥有独特的多重极端条件，孕育了具有重大生物技术潜力的多种微生物。这是由于基因组适应极端条件导致了代谢改变，例如产生独特的胞外多糖。从南极洲欺骗岛分离出的地衣芽孢杆菌F2LB，对其胞外多糖（EPS）的结构和生物技术潜力进行了评估。基因组分析揭示了EPS生物合成、抗紫外线和热适应基因。该EPS具有假定的葡糖半乳甘露聚糖主链，主要由糖摩尔比为甘露糖：葡萄糖：木糖：岩藻糖（1:0.7:0.4:0.3）以及少量其他糖类组成。光谱分析确定了关键糖苷键，形态学显示其表面粗糙、无孔。在功能方面，EPS表现出强大的热稳定性（T 255°C）、显著的抗氧化活性（约100%的过氧化氢清除率；DPPH：75.5%）、良好的乳化能力（橄榄油中为90%），以及持水能力（238.7%）和持油能力（384.7%）超过商业黄原胶。这些发现突显了其作为可持续食品添加剂的潜力。
