Vargas Bruna Krieger, Fabricio Mariana Fensterseifer, Schmidt Luana, Martins Caroline Carboni, Viçozzi Gabriel Pedroso, Rodrigues Eliseu, Augusti Paula Rossini, Ayub Marco Antônio Záchia
Food Science and Technology Institute, Brazil.
Laboratory of Toxicology (LATOX), Department of Analysis, Faculty of Pharmacy, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil, Av. Bento Gonçalves, 9500, ZC 91501-970 Porto Alegre, Rio Grande do Sul, Brazil.
Food Chem. 2025 Nov 15;492(Pt 3):145590. doi: 10.1016/j.foodchem.2025.145590. Epub 2025 Jul 17.
Kombucha is known for its high phenolic contents, associated with its biological properties. Here, we evaluated the phenolic profile of a standardized green tea kombucha and the antioxidant activities of three concentrations (25, 125, and 250 μL•mL) both in vitro and in vivo models. A total of 35 phenolic compounds were identified by HPLC-DAD-MS/MS analysis, focused on the quantification of (Epi)gallocatechin gallate and its derivatives. The three evaluated doses of kombucha presented no in vivo toxicity and reduced the generation of reactive species and lipid peroxidation levels induced by HO. Doses of 125 and 250 μL•mL increased the SOD fluorescence in C. elegans. However, no kombucha doses attenuated the loss of survival of C. elegans induced by HO. Results suggest that drinking 600 mL per day of our tailor-made kombucha might be the feasible amount of kombucha ingestion able to perform antioxidant protection without showing toxicity.
康普茶以其高酚含量及其生物学特性而闻名。在此，我们评估了标准化绿茶康普茶的酚类成分以及三种浓度（25、125和250 μL•mL）在体外和体内模型中的抗氧化活性。通过HPLC-DAD-MS/MS分析共鉴定出35种酚类化合物，重点是对（表）没食子儿茶素没食子酸酯及其衍生物进行定量。所评估的三种康普茶剂量在体内均无毒性，并减少了由HO诱导的活性物质生成和脂质过氧化水平。125和250 μL•mL的剂量增加了秀丽隐杆线虫中的超氧化物歧化酶（SOD）荧光。然而，没有康普茶剂量能减轻HO诱导的秀丽隐杆线虫的生存损失。结果表明，每天饮用600 mL我们特制的康普茶可能是可行的康普茶摄入量，既能起到抗氧化保护作用又不显示毒性。
