Guo Zhongshang, Chen Huihui, Hu Ruiling, Wang Jiawei, Wu Miao, Wu Yinghua, Qiang Tinghui, Mou Huan, Du Xingguo, Gao Fei, Guo Shaobo, Zhou Xinli
Department of Osteoarticular Surgery Department, Hanzhong Central Hospital, Hanzhong, 723000, Shaanxi, China.
Shaanxi Key Laboratory of Catalysis, School of Chemical & Environment Science, Shaanxi University of Technology, Hanzhong, 723000, Shaanxi, China.
Sci Rep. 2025 Jul 23;15(1):26780. doi: 10.1038/s41598-025-93691-1.
In recent years, the widespread use of antibiotics has led to the emergence of numerous drug-resistant bacteria, posing a severe threat to both human health and the economy. As a result, it is imperative to develop efficient antibacterial agents that do not induce drug resistance. This study employed layer-by-layer assembly technology to prepare ZnFeO@ZnS/CuS (ZZC) nanocomposites Gram-negative Escherichia coli (E. coli), Gram-positive Staphylococcus aureus (S. aureus) and drug-resistant Salmonella (T-Salmonella) were utilized as test bacteria to investigate the antibacterial effectiveness and mechanism of ZZC. The findings demonstrated that, the MIC of the ZZC against E. coli, S. aureus and T-Salmonella were 50, 60 and 80 μg/mL, respectively; At a material concentration of 200 μg/mL and a reaction time of 80 min, ZZC demonstrated a bacteriostatic rate of 99.99% against the three tested bacteria. The nano-composite can disrupt cell walls and plasma membranes and effectively and resulting in bacterial rupture and demise. Furthermore, the nano-composite displayed strong biocompatibility and was also able to heal mixed bacterial-induced wound infections and essentially eliminated the bacterial burden after 9 days, and also exhibited excellent antimicrobial activity in vivo. The results also indicate significant potential for its application in medical materials and other areas of research.
近年来，抗生素的广泛使用导致了大量耐药细菌的出现，对人类健康和经济都构成了严重威胁。因此，开发不会诱导耐药性的高效抗菌剂势在必行。本研究采用层层组装技术制备了ZnFeO@ZnS/CuS（ZZC）纳米复合材料，以革兰氏阴性大肠杆菌（E. coli）、革兰氏阳性金黄色葡萄球菌（S. aureus）和耐药沙门氏菌（T-沙门氏菌）作为测试细菌，研究ZZC的抗菌效果和作用机制。研究结果表明，ZZC对大肠杆菌、金黄色葡萄球菌和T-沙门氏菌的最低抑菌浓度分别为50、60和80μg/mL；在材料浓度为200μg/mL、反应时间为80分钟时，ZZC对三种测试细菌的抑菌率为99.99%。该纳米复合材料可破坏细胞壁和细胞膜，有效导致细菌破裂和死亡。此外，该纳米复合材料具有很强的生物相容性，还能够治愈混合细菌引起的伤口感染，在9天后基本消除细菌负荷，并且在体内也表现出优异的抗菌活性。结果还表明其在医用材料等研究领域具有巨大的应用潜力。
