Zhang Chunhong, Sheng Qinglin, Wang Yuanqi, Shen Qi, Zhai Yifei, Hu Dawei, Zhang Nanjia, Wang Ziyuan, Yin Xuebin, Li Dan, Chen Youtao
Institute of Food Science and Technology, CAAS, Beijing, China.
Guangdong Provincial Key Laboratory of Intelligent Port Security Inspection, Guangzhou, China.
Front Microbiol. 2025 Jul 10;16:1586864. doi: 10.3389/fmicb.2025.1586864. eCollection 2025.
The integrated analysis of gut and oral microbiota and their metabolites helps elucidate key factors affecting sleep disorders in populations and provides research insights for understanding sleep regulation mechanisms.
Based on a cross-sectional study design, this research combined 16S sequencing and untargeted metabolomics to investigate lifestyle habits and physical conditions of 165 adult male subjects, systematically examining characteristics of gut and oral microbiota and their metabolites.
Analysis of gut microbiota revealed significantly reduced microbial diversity in the insomnia group, with predominant phyla being , , and . At the genus level, the abundance of Blautia was significantly elevated. Gut metabolite analysis showed significant enrichment in metabolic pathways such as "phenylalanine, tyrosine, and tryptophan biosynthesis." Regarding oral microbiota, no significant difference in diversity was observed between sleepless and normal groups. At the genus level, the sleepless group showed significantly decreased abundance of and increased abundance of . Metabolite analysis indicated significant correlation between the sleepless group and metabolic pathways such as "pantothenate and CoA biosynthesis."
This study compared differences in gut and oral microbiota and metabolites between sleepless and normal groups, identifying potential biomarkers for insomnia, including gut , aromatic amino acid metabolites, salivary and , and pantothenate-related metabolites. These findings provide important multi-omics data for investigating the pathological mechanisms of insomnia. We have made changes according to the requirements, please adjust according to the standard.
对肠道和口腔微生物群及其代谢产物进行综合分析，有助于阐明影响人群睡眠障碍的关键因素，并为理解睡眠调节机制提供研究思路。
基于横断面研究设计，本研究结合16S测序和非靶向代谢组学，对165名成年男性受试者的生活习惯和身体状况进行调查，系统检测肠道和口腔微生物群及其代谢产物的特征。
肠道微生物群分析显示，失眠组的微生物多样性显著降低，优势菌门为 、 和 。在属水平上，布劳特氏菌属的丰度显著升高。肠道代谢产物分析表明，“苯丙氨酸、酪氨酸和色氨酸生物合成”等代谢途径显著富集。关于口腔微生物群，失眠组和正常组之间在多样性上未观察到显著差异。在属水平上，失眠组显示 丰度显著降低， 丰度增加。代谢产物分析表明，失眠组与“泛酸和辅酶A生物合成”等代谢途径之间存在显著相关性。
本研究比较了失眠组和正常组在肠道和口腔微生物群及代谢产物方面的差异，确定了失眠的潜在生物标志物，包括肠道 、芳香族氨基酸代谢产物、唾液 和 以及泛酸相关代谢产物。这些发现为研究失眠的病理机制提供了重要的多组学数据。我们已根据要求进行了修改，请根据标准进行调整。
