Huh Jung, Jeong Seong-Ho, Dhong Eun-Sang, Han Seung-Kyu, Moon Kyung-Chul
Korea University College of Medicine, Seoul 02841, Republic of Korea.
Department of Plastic Surgery, Korea University College of Medicine, Seoul 02841, Republic of Korea.
Bioengineering (Basel). 2025 Jul 5;12(7):736. doi: 10.3390/bioengineering12070736.
We developed biomimetic full-thickness artificial skin using stromal vascular fraction (SVF) cells and autologous keratinocytes for the dermal and epidermal layers of skin, respectively. Full-thickness artificial skin scaffolds were fabricated using 4% porcine collagen and/or elastin in a low-temperature three-dimensional printer. Two types of scaffolds with collagen-to-elastin ratios of 100:0 and 100:4 were printed and compared. The scaffolds were analyzed for collagenase degradation, tensile strength, and structural features using scanning electron microscopy. By 24 h, the collagen-only scaffolds showed gradual degradation, and the collagen-elastin scaffolds retained the highest structural integrity but were not degraded. In the tensile strength tests, the collagen-only scaffolds exhibited a tensile strength of 2.2 N, while the collagen-elastin scaffolds showed a tensile strength of 4.2 N. Cell viability tests for keratinocytes displayed an initial viability of 89.32 ± 3.01% on day 1, which gradually increased to 97.22 ± 4.99% by day 7. Similarly, SVF cells exhibited a viability of 93.68 ± 1.82% on day 1, which slightly improved to 97.12 ± 1.64% on day 7. This study presents a novel strategy for full-thickness artificial skin development, combining SVF and keratinocytes with an optimized single collagen scaffold and a gradient pore-density structure.
我们分别使用基质血管成分（SVF）细胞和自体角质形成细胞开发了用于皮肤真皮层和表皮层的仿生全层人工皮肤。使用4%的猪胶原蛋白和/或弹性蛋白在低温三维打印机中制造全层人工皮肤支架。打印并比较了两种胶原蛋白与弹性蛋白比例为100:0和100:4的支架。使用扫描电子显微镜对支架进行胶原酶降解、拉伸强度和结构特征分析。到24小时时，仅含胶原蛋白的支架显示出逐渐降解，而含胶原蛋白-弹性蛋白的支架保持了最高的结构完整性但未降解。在拉伸强度测试中，仅含胶原蛋白的支架表现出2.2 N的拉伸强度，而含胶原蛋白-弹性蛋白的支架显示出4.2 N的拉伸强度。角质形成细胞的细胞活力测试显示，第1天的初始活力为89.32±3.01%，到第7天逐渐增加到97.22±4.99%。同样，SVF细胞在第1天的活力为93.68±1.82%，在第7天略有提高至97.12±1.64%。本研究提出了一种全层人工皮肤开发的新策略，将SVF和角质形成细胞与优化的单一胶原蛋白支架和梯度孔密度结构相结合。
