Očenáš Peter, Baloga Matej, Valko-Rokytovská Marcela, Ivašková Sonja
Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Life (Basel). 2025 Jul 20;15(7):1141. doi: 10.3390/life15071141.
Due to advances in molecular technologies and the expanding knowledge of biomarkers, their use in patient screening, diagnosis, prognosis, and targeted therapy is continuously increasing. Biomarker characteristics play a crucial role across all areas of medical research/practice. Biomarkers often reflect changes in the biochemical composition of biofluids, which can be qualitatively and quantitatively analyzed using methods such as high-performance liquid chromatography (HPLC) at various stages of clinical intervention. This study focuses on establishing physiological reference ranges for selected biochemical and metabolomic indicators by analyzing blood serum samples from domestic sheep. A total of sixty samples are examined using standard biochemical assays and HPLC, resulting in the determination of experimental reference values for twenty-one biochemical and eight metabolomic parameters. Reliable and reproducible preclinical testing is essential before any diagnostic method can be introduced into clinical use. A thorough understanding of the safety and efficacy of such methods in animal models is a prerequisite for initiating human trials. Species selection and the definition of physiological biomarker ranges are therefore critical components in the development of effective preclinical protocols. This work contributes to the foundation needed for further clinical testing by establishing reference values for relevant biomarkers in a commonly used animal model.
由于分子技术的进步以及对生物标志物认识的不断扩展，它们在患者筛查、诊断、预后和靶向治疗中的应用持续增加。生物标志物特征在医学研究/实践的各个领域都起着至关重要的作用。生物标志物通常反映生物流体生化组成的变化，在临床干预的各个阶段，可使用高效液相色谱法（HPLC）等方法对其进行定性和定量分析。本研究旨在通过分析家羊血清样本，为选定的生化和代谢组学指标建立生理参考范围。使用标准生化检测方法和HPLC对总共60个样本进行检测，确定了21个生化参数和8个代谢组学参数的实验参考值。在任何诊断方法引入临床使用之前，可靠且可重复的临床前测试至关重要。全面了解此类方法在动物模型中的安全性和有效性是启动人体试验的先决条件。因此，物种选择和生理生物标志物范围的定义是有效临床前方案开发的关键组成部分。这项工作通过在常用动物模型中建立相关生物标志物的参考值，为进一步的临床试验奠定了基础。
