比较转录组和代谢组分析为胡萝卜主根发育和生物活性化合物生物合成的分子机制提供了新见解。

Comparative Transcriptome and Metabolome Analyses Provide New Insights into the Molecular Mechanisms Underlying Taproot Development and Bioactive Compound Biosynthesis in vahl.

作者信息

Tang Meiqiong, Liang Chunying, Peng Yude, He Hong, Wei Fan, Hu Ying, Lin Yang, Tang Chunfeng, Li Gang, Li Linxuan

机构信息

Guangxi Engineering Research Center of TCM Resource Intelligent Creation, Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, National Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.

College of Agriculture, Guangxi University, Nanning 530004, China.

出版信息

Genes (Basel). 2025 Jun 30;16(7):784. doi: 10.3390/genes16070784.

DOI:10.3390/genes16070784

PMID:40725441

Abstract

BACKGROUND

vahl is a famous Chinese medicinal plant. The root is the main organ accumulating bioactive compounds, and its development is directly related to the yield and quality of the harvested However, the molecular mechanisms underlying the bioactive compound biosynthesis occurring during the root development of are unknown.

METHOD

Transcriptome and widely targeted metabolome analyses were performed to investigate gene expression and metabolite variation during the development of taproots.

RESULTS

A total of 3792 differentially expressed genes (DEGs) were identified between the one- and three-year-old taproots; they are related to circadian rhythm-plant, phenylpropanoid biosynthesis, starch and sucrose metabolism, and plant-pathogen interaction pathways. In total, 119 differentially accumulated metabolites (DAMs) were identified between the one- and three-year-old taproots, including flavonols, phenolic acids, and coumarins compounds. Integrative transcriptome and metabolome analyses revealed a significant correlation between 172 DEGs and 21 DAMs; they were predominantly enriched for processes associated with phenylpropanoid biosynthesis, flavonoid biosynthesis, plant hormone signal transduction, and stilbenoid, diarylheptanoid, and ginerol biosynthesis. In addition, 26 DEGs were identified to be significantly correlated with the DAMs that accumulated in the phenylpropanoid biosynthesis pathway, and these DEGs may be the key genes for the biosynthesis of active compounds.

CONCLUSIONS

The phenylpropanoid biosynthesis pathway plays a dual role in both development and bioactive compound synthesis in taproots. These findings provide a molecular regulatory network in the relationships between taproot development and the accumulation of secondary metabolites. The identification of candidate genes and pathways provides a genetic resource for quality control and future molecular breeding in .

摘要

背景

娃儿藤是一种著名的中国药用植物。根是积累生物活性化合物的主要器官，其发育直接关系到收获物的产量和质量。然而，娃儿藤根发育过程中生物活性化合物生物合成的分子机制尚不清楚。

方法

进行转录组和广泛靶向代谢组分析，以研究娃儿藤主根发育过程中的基因表达和代谢物变化。

结果

在一年生和三年生娃儿藤主根之间共鉴定出3792个差异表达基因（DEG）；它们与昼夜节律-植物、苯丙烷生物合成、淀粉和蔗糖代谢以及植物-病原体相互作用途径有关。在一年生和三年生娃儿藤主根之间总共鉴定出119种差异积累代谢物（DAM），包括黄酮醇、酚酸和香豆素类化合物。转录组和代谢组综合分析显示172个DEG与21个DAM之间存在显著相关性；它们主要富集于与苯丙烷生物合成、类黄酮生物合成、植物激素信号转导以及芪类、二芳基庚烷类和姜醇生物合成相关的过程。此外，鉴定出26个DEG与苯丙烷生物合成途径中积累的DAM显著相关，这些DEG可能是娃儿藤活性化合物生物合成的关键基因。