Singhla Tawatchai, Boonsri Kittikorn, Kreausukon Khwanchai, Modethed Wittawat, Pringproa Kidsadagon, Sthitmatee Nattawooti, Punyapornwithaya Veerasak, Vinitchaikul Paramintra
Ruminant Clinic, Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
Center of Excellence in Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
Vet Sci. 2022 Apr 18;9(4):194. doi: 10.3390/vetsci9040194.
Understanding molecular epidemiology is essential for the improvement of lumpy skin disease (LSD) eradication and control strategies. The objective of this study was to perform a molecular characterization and phylogenetic analysis of lumpy skin disease virus (LSDV) isolated from dairy cows presenting LSD-like clinical signs in northern Thailand. The skin nodules were collected from 26 LSD-suspected cows involved in six outbreaks during the period from July to September of 2021. LSDVs were confirmed from clinical samples using the polymerase chain reaction (PCR). The PCR-positive samples were subsequently amplified and sequenced using a G-protein-coupled chemokine receptor (GPCR) gene for molecular characterization and phylogenetic analyses. All 26 samples were positive for LSDV by PCR. A phylogenetic analysis indicated that the 24 LSDV isolates obtained from cattle in northern Thailand were closely related to other LSDV sequences acquired from Asia (China, Hong Kong, and Vietnam). On the other hand, two LSDV isolates of the cows presenting LSD-like clinical signs after vaccination were clustered along with LSDV Neethling-derived vaccines. The outcomes of this research will be beneficial in developing effective control strategies for LSDV.
了解分子流行病学对于改进牛结节性皮肤病（LSD）的根除和控制策略至关重要。本研究的目的是对从泰国北部出现类似LSD临床症状的奶牛中分离出的牛结节性皮肤病病毒（LSDV）进行分子特征分析和系统发育分析。在2021年7月至9月期间，从6起疫情中涉及的26头疑似LSD的奶牛身上采集了皮肤结节。使用聚合酶链反应（PCR）从临床样本中确认了LSDV。随后，对PCR阳性样本进行扩增，并使用G蛋白偶联趋化因子受体（GPCR）基因进行测序，以进行分子特征分析和系统发育分析。所有26个样本经PCR检测均为LSDV阳性。系统发育分析表明，从泰国北部牛身上获得的24株LSDV分离株与从亚洲（中国、中国香港和越南）获得的其他LSDV序列密切相关。另一方面，两株接种疫苗后出现类似LSD临床症状的奶牛的LSDV分离株与源自Neethling的LSDV疫苗聚集在一起。本研究结果将有助于制定有效的LSDV控制策略。
