Pławińska-Czarnak Joanna, Majewska Alicja, Zarzyńska Joanna Magdalena, Kaba Jarosław, Bagnicka Emilia
Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland.
Department of Physiology Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland.
Viruses. 2025 Jul 3;17(7):944. doi: 10.3390/v17070944.
Caprine arthritis and encephalitis (CAE), caused by small ruminant lentivirus (SRLV), is a key disease of goats, with chronic inflammation of joints and brain symptoms leading to losses in milk production and animal trade. In this study, we analyzed gene expressions in the milk somatic cells (MSCs) of seropositive (SRLV-SP) and seronegative (SRLV-SN) goats to identify transcriptomic changes using a non-invasive sampling method.
This study was conducted on goats of two Polish breeds (Polish Improved White and Polish Improved Fawn), which were kept at the Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, during their first lactation. MSCs were isolated from milk, and gene expression was analyzed using the Goat Gene Expression Microarray. The results were verified by RT-qPCR for five genes (, , , , ). Statistical analysis was performed in GeneSpring 12 software.
Microarrays showed reduced expression of , , , , and genes in SRLV-SP goats. RT-qPCR confirmed changes for , , and . Functional analysis indicated associations with immune processes and HIV-like pathways.
The results suggest that SRLV induces transcriptomic perturbations, especially in immunity-related genes. MSCs are an effective model for non-invasive studies, and further studies may support strategies for combating CAE.
由小反刍兽慢病毒（SRLV）引起的山羊关节炎脑炎（CAE）是山羊的一种关键疾病，会导致关节慢性炎症和脑部症状，从而造成产奶量下降和动物贸易损失。在本研究中，我们使用非侵入性采样方法分析了血清阳性（SRLV-SP）和血清阴性（SRLV-SN）山羊的乳体细胞（MSC）中的基因表达，以确定转录组变化。
本研究以两个波兰品种（波兰改良白山羊和波兰改良黄褐山羊）的山羊为对象，它们在首次泌乳期间饲养于波兰科学院遗传与动物生物技术研究所。从乳汁中分离出乳体细胞，并使用山羊基因表达微阵列分析基因表达。通过RT-qPCR对五个基因（、、、、）的结果进行了验证。在GeneSpring 12软件中进行统计分析。
微阵列显示SRLV-SP山羊中、、、、和基因的表达降低。RT-qPCR证实了、和的变化。功能分析表明与免疫过程和HIV样途径有关。
结果表明SRLV会诱导转录组扰动，尤其是在免疫相关基因中。乳体细胞是非侵入性研究的有效模型，进一步的研究可能支持对抗CAE的策略。
