Roamcharern Napaporn, Punnabhum Panida, Seib F Philipp, Rattray Zahra
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde 161 Cathedral St. Glasgow G4 0RE Scotland UK
Fraunhofer Institute for Molecular Biology and Applied Ecology, Branch Bioresources Ohlebergsweg 12 35392 Giessen Germany.
Nanoscale Adv. 2025 Jul 21. doi: 10.1039/d5na00365b.
Silk fibroin is a promising material for nanocarrier-based drug delivery applications due to its biocompatibility, biodegradability, and mechanical properties, which can be fine-tuned through processing conditions. In this study, we explore the impact of Ca and K inclusion on the morphology of silk nanoparticles and evaluate the short- and long-term stability of silk nanoparticles formed by antisolvent precipitation in deionized water and sodium phosphate buffer. Using advanced electric asymmetric flow field-flow fractionation multiplexed with online detectors (EAF4-UV-MALS-DLS) and orthogonal analytics (DLS, ELS, NTA, FE-SEM), we analyze the physicochemical attributes of silk nanoparticles. We find significant differences in nanoparticle architecture and stability in different buffers, with notable differences in particle size ( and ), charge, and shape measured over 56 days. Notably, nanoparticles formulated with 0.7 mg Ca and 1.1 mg K maintained superior physicochemical stability, making them promising candidates for future nanocarrier-based applications.
由于丝素蛋白具有生物相容性、生物可降解性和机械性能，且可通过加工条件进行微调，因此它是用于基于纳米载体的药物递送应用的一种很有前景的材料。在本研究中，我们探究了钙（Ca）和钾（K）的加入对丝纳米颗粒形态的影响，并评估了在去离子水和磷酸钠缓冲液中通过反溶剂沉淀法形成的丝纳米颗粒的短期和长期稳定性。我们使用与在线检测器联用的先进的电不对称流场-流分级法（EAF4-UV-MALS-DLS）和正交分析方法（DLS、ELS、NTA、FE-SEM），分析了丝纳米颗粒的物理化学属性。我们发现，在不同缓冲液中，纳米颗粒的结构和稳定性存在显著差异，在56天的时间里，颗粒大小（ 和 ）、电荷和形状都有明显差异。值得注意的是，含有0.7毫克钙和1.1毫克钾的纳米颗粒保持了优异的物理化学稳定性，使其成为未来基于纳米载体应用的有前景的候选材料。
