还原态蛋白质中半胱氨酰残基便捷的S-2-氨乙基化反应。
A convenient S-2-aminoethylation of cysteinyl residues in reduced proteins.
作者信息
Okazaki K, Yamada H, Imoto T
出版信息
Anal Biochem. 1985 Sep;149(2):516-20. doi: 10.1016/0003-2697(85)90607-4.
PMID:4073506
Abstract
The cysteinyl residues in proteins had been S-2-aminoethylated with ethylenimine quantitatively. However, ethylenimine is now listed as a carcinogen and is no longer commercially available. A method of converting cysteinyl residues to S-2-aminoethyl derivatives quantitatively using 2-bromoethylamine under mild conditions was developed here.
摘要
蛋白质中的半胱氨酰残基已被乙烯亚胺定量地S-2-氨乙基化。然而，乙烯亚胺现在被列为致癌物，已不再商业化供应。本文开发了一种在温和条件下使用2-溴乙胺将半胱氨酰残基定量转化为S-2-氨乙基衍生物的方法。
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