Nowak Weronika, Kołodziejczyk-Czepas Joanna, Liudvytska Oleksandra, Tybura-Sawicka Marzena, Krzemińska Emilia, Puła Anna, Treliński Jacek
Department of Hemostasis Disorders, Medical University of Lodz, Pabianicka 62, Łódź, 93-513, Poland.
Department of Hematooncology, Copernicus Memorial Hospital in Lodz, Pabianicka 62, Łódź, Poland.
Thromb J. 2025 Jul 30;23(1):77. doi: 10.1186/s12959-025-00762-4.
Antiphospholipid antibodies (aPLs) are detected in 1-5% of the general population. They include lupus anticoagulant (LAC), anticardiolipin antibodies (aCL) and anti-β2-glycoprotein I antibodies (aβ2GPI). APL increases thrombotic risk, but the pathogenesis of this effect is not fully understood.
The aim of this study was to evaluate oxidative and nitrosative stress biomarkers and their relation to certain rotational thromboelastometry (ROTEM) parameters as a risk factor for thrombosis in 32 patients in whom the presence of antiphospholipid antibodies was confirmed, but who had never experienced a thrombosis event (Group 1) in order to rule out any impact of thrombosis on stress parameters. The parameters were also assessed in a group of 23 healthy volunteers (Group 2).
To assess FRAP and thiol groups we used colorimetric method. The level of protein carbonylation, total pool of 3-nitrotyrosine in plasma proteins, 3-nitrotyrosine-containing fibrinogen as well as the acetyl-lysine-containing fibrinogen were estimated by ELISA. Lipid hydroperoxides were detected using the ferric-xylenol orange hydroperoxide assay. Additionally four ROTEM tests, i.e. INTEM, EXTEM, FIBTEM and APTEM, were performed. In statistical analysis the Mann-Whitney U-test, Student's t-test and logistic regression were used.
TBARS (p = 0,002), LOOH (p = 0,035) and carbonyl groups (p = 0,018) were markedly higher in Group 1 compared to Group 2. Also the acetyl-lysine-containing fibrinogen were significantly higher in Group 1 (p = 0,0028). Other biomarkers did not differ markedly between the studied groups. The obtained results of ROTEM, were not consistent and did not clearly indicate hypercoagulable state.
Study confirms increased levels of oxidative biomarkers in patients in whom the presence of antiphospholipid antibodies was confirmed, but who had never experienced a thrombosis event. Oxidative stress may an important role in the pathogenesis of APS and is not secondary to thrombosis.
在普通人群中，抗磷脂抗体（aPLs）的检测率为1% - 5%。它们包括狼疮抗凝物（LAC）、抗心磷脂抗体（aCL）和抗β2糖蛋白I抗体（aβ2GPI）。抗磷脂抗体增加血栓形成风险，但其作用机制尚未完全明确。
本研究旨在评估32例确诊存在抗磷脂抗体但从未发生过血栓事件的患者（第1组）的氧化应激和亚硝化应激生物标志物及其与某些旋转血栓弹力图（ROTEM）参数的关系，以作为血栓形成的危险因素，从而排除血栓形成对应激参数的任何影响。同时，对23名健康志愿者（第2组）进行了这些参数的评估。
我们采用比色法评估FRAP和巯基基团。通过酶联免疫吸附测定（ELISA）法评估血浆蛋白中的蛋白质羰基化水平、3 - 硝基酪氨酸的总量、含3 - 硝基酪氨酸的纤维蛋白原以及含乙酰赖氨酸的纤维蛋白原。使用铁 - 二甲苯酚橙过氧化氢测定法检测脂质过氧化氢。此外，还进行了四项ROTEM检测，即INTEM、EXTEM、FIBTEM和APTEM。在统计分析中，使用了曼 - 惠特尼U检验、学生t检验和逻辑回归分析。
与第2组相比，第1组的硫代巴比妥酸反应物（TBARS，p = 0.002）、脂质过氧化氢（LOOH，p = 0.035）和羰基基团（p = 0.018）明显更高。第1组中含乙酰赖氨酸的纤维蛋白原也显著更高（p = 0.0028）。其他生物标志物在研究组之间没有明显差异。获得的ROTEM结果不一致，未明确显示高凝状态。
研究证实，在确诊存在抗磷脂抗体但从未发生过血栓事件的患者中，氧化应激生物标志物水平升高。氧化应激可能在抗磷脂综合征的发病机制中起重要作用，而非继发于血栓形成。
