Ren Meifeng, Zhang Yuying, Wang Yifei, Duan Yuanpeng, Gao Yue, Liu Zhongfang, Zhang Pengjiu, He Lifei, Fan Renjun, Zhou Xuguo, Yang Jing
College of Plant Protection, Shanxi Agricultural University, Shanxi Key Laboratory of Integrated Pest Management in Agriculture, Taiyuan, Shanxi, China.
Department of Entomology, School of Integrative Biology, College of Liberal Arts & Sciences, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
Sci Data. 2025 Aug 1;12(1):1342. doi: 10.1038/s41597-025-05683-5.
The hawthorn spider mite, Amphitetranychus viennensis, is a major pest of orchards and ornamentals in the Palaearctic region, with adaptability and acaricide resistance. The lack of high-quality genomic resources limits understanding of its detoxification mechanisms and the development of RNAi-based pest control strategies. In this study, we utilized Illumina, Pacific Biosciences (PacBio), and Hi-C sequencing technologies to assemble a chromosome-level reference genome of A. viennensis. The assembled genome spans 141.96 Mb, with a contig N50 of 1.35 Mb. BUSCO analysis confirmed a high level of completeness, covering 91.6% of annotated genes. The assembly includes 50.97 Mb of repetitive sequences, representing 35.93% of the genome, and annotates 13,968 protein-coding genes. Using Hi-C sequencing, we anchored 47 contigs to three chromosomes, accounting for 97.27% of the estimated nuclear genome and achieving a contig N50 of 45.83 Mb. This high-quality genome assembly provides a valuable foundation for evolutionary and genomic research on spider mites, while also serving as a genetic resource to inform molecular control strategies and support sustainable pest management.
山楂叶螨（Amphitetranychus viennensis）是古北区果园和观赏植物的主要害虫，具有适应性和杀螨剂抗性。缺乏高质量的基因组资源限制了对其解毒机制的了解以及基于RNA干扰的害虫防治策略的开发。在本研究中，我们利用Illumina、PacBio和Hi-C测序技术组装了山楂叶螨的染色体水平参考基因组。组装的基因组跨度为141.96 Mb，重叠群N50为1.35 Mb。BUSCO分析证实了其高度的完整性，覆盖了91.6%的注释基因。该组装包含50.97 Mb的重复序列，占基因组的35.93%，并注释了13,968个蛋白质编码基因。利用Hi-C测序，我们将47个重叠群锚定到三条染色体上，占估计核基因组的97.27%，重叠群N50达到45.83 Mb。这种高质量的基因组组装为叶螨的进化和基因组研究提供了宝贵的基础，同时也作为一种遗传资源，为分子控制策略提供信息并支持可持续的害虫管理。
