1,8-二硝基芘诱导的大鼠纤维肉瘤中K-ras及ras家族以外的癌基因的激活

Activation of K-ras and oncogenes other than ras family in rat fibrosarcomas induced by 1,8-dinitropyrene.

作者信息

Ochiai M, Nagao M, Tahira T, Ishikawa F, Hayashi K, Ohgaki H, Terada M, Tsuchida N, Sugimura T

出版信息

Cancer Lett. 1985 Nov;29(2):119-25. doi: 10.1016/0304-3835(85)90150-8.

Abstract

Oncogenes of fibrosarcomas of rats, induced by subcutaneous injection of 1,8-dinitropyrene (1,8-DNP), were examined by NIH 3T3 cell transfection assay and Southern blot analysis. Transformants containing rat specific repetitive sequences were obtained with DNAs of 4 fibrosarcomas, 1,8-DNP1, 1,8-DNP2, 1,8-DNP3 and 1,8-DNP7. A transformant, 1,8-DNP2-2, induced by DNA of a fibrosarcoma, 1,8-DNP2, and 7 secondary transformants derived from it contained rat K-ras sequences. Another transformant, 1,8-DNP2-1, induced by the same sarcoma did not have a ras family oncogene. This indicates that the sarcoma, 1,8-DNP2, has at least 2 transforming genes. The transforming genes of 6 other transformants derived from 3 sarcomas did not contain ras family or neu transforming genes.

摘要

通过NIH 3T3细胞转染试验和Southern印迹分析，对皮下注射1,8 - 二硝基芘（1,8 - DNP）诱导的大鼠纤维肉瘤的癌基因进行了检测。用4个纤维肉瘤（1,8 - DNP1、1,8 - DNP2、1,8 - DNP3和1,8 - DNP7）的DNA获得了含有大鼠特异性重复序列的转化体。由纤维肉瘤1,8 - DNP2的DNA诱导产生的一个转化体1,8 - DNP2 - 2及其衍生的7个二级转化体含有大鼠K - ras序列。由同一肉瘤诱导产生的另一个转化体1,8 - DNP2 - 1没有ras家族癌基因。这表明肉瘤1,8 - DNP2至少有2个转化基因。来自3个肉瘤的其他6个转化体的转化基因不包含ras家族或neu转化基因。