Borah Pulakesh, Baruah Diksha J, Mridha Prosenjit, Duarah Rituparna, Baishya Rinku, Das Manash R
Materials Sciences Group, Coal, Energy and Materials Sciences Division, CSIR-North East Institute of Science and Technology, Jorhat, Assam 785006, India.
Academy of Scientific and Innovative Research, Ghaziabad 201002, India.
ACS Omega. 2025 Jul 21;10(29):32269-32281. doi: 10.1021/acsomega.5c04246. eCollection 2025 Jul 29.
The inherent reducing properties of borophene nanosheets (BNSs) were harnessed to facilitate the reduction of gold ions, leading to the synthesis of gold nanoparticle-decorated borophene nanosheets (Au@BNSs) via an eco-friendly, green approach and eliminating the need for toxic external reducing agents. This innovative synthesis method addresses the limitations of conventional glucose monitoring systems, which often rely on expensive reagents and sophisticated equipment. The synthesized Au@BNSs nanocomposite demonstrated dual enzyme-mimetic activity, replicating both glucose oxidase (GOx) and peroxidase (POD) and facilitating efficient colorimetric glucose detection. Comprehensive characterization using X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed infrared spectroscopy (FTIR), energy dispersive X-ray (EDX), X-ray photoelectron spectroscopy (XPS), and dynamic light scattering (DLS) studies confirmed the successful decoration of BNSs with gold nanoparticles with an average particle size of 8.5 nm. The nanocomposite exhibited high stability, biocompatibility, and efficient catalytic activity, enabling selective glucose detection with a detection limit of 0.05 mM within a linear range of 30-330 μM. The practical applicability of the Au@BNSs-based colorimetric sensor assay was demonstrated by using human serum samples as real samples. Thus, Au@BNSs serve as a robust platform for biosensing applications, providing an innovative and efficient approach to glucose monitoring.
利用硼烯纳米片（BNSs）固有的还原特性来促进金离子的还原，通过一种环保、绿色的方法合成了金纳米颗粒修饰的硼烯纳米片（Au@BNSs），无需使用有毒的外部还原剂。这种创新的合成方法克服了传统葡萄糖监测系统的局限性，传统系统通常依赖昂贵的试剂和复杂的设备。合成的Au@BNSs纳米复合材料表现出双酶模拟活性，模拟葡萄糖氧化酶（GOx）和过氧化物酶（POD），并促进高效的比色法葡萄糖检测。使用X射线衍射（XRD）、透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）、能量色散X射线（EDX）、X射线光电子能谱（XPS）和动态光散射（DLS）研究进行的综合表征证实，金纳米颗粒成功修饰了BNSs，平均粒径为8.5 nm。该纳米复合材料表现出高稳定性、生物相容性和高效催化活性，能够在30 - 330 μM的线性范围内进行选择性葡萄糖检测，检测限为0.05 mM。通过使用人血清样本作为实际样本，证明了基于Au@BNSs的比色传感器测定法的实际适用性。因此，Au@BNSs作为一种强大的生物传感应用平台，为葡萄糖监测提供了一种创新且高效的方法。
