Wan Xiaoming, Liu Weimeng, Li Zichan, Lu Fangwei, Wang Can, Li Xia, Liu Guixia
School of Life Sciences, Hebei University, Baoding, 071002, China.
Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding, 071002, People's Republic of China.
Curr Microbiol. 2025 Aug 4;82(9):435. doi: 10.1007/s00284-025-04410-y.
The aim of this study was to investigate the correlations between the morphology and microbial community structure of Medicago ruthenica and soil environmental factors. Soil samples were collected from M. ruthenica and from rhizosphere and non-rhizosphere soils under typical grasslands (C1), desertified grasslands (C2) and wetland meadows (C3). The morphological characteristics, soil physicochemical properties and enzyme activities of M. ruthenica varied significantly (P < 0.05) across the different habitats. Branch length and stem diameter were greatest in the C2 plot, and leaf area and leaf volume were greatest in the C3 plot. The contents of organic matter, alkaline dissolved nitrogen and quick-acting potassium, as well as the activities of soil sucrase and soil phosphatase, were ranked in the order of C3 > C1 > C2. The microbial community composition also varies by habitat. The dominant bacterial phyla were Proteobacteria, Actinobacteria, and Acidobacteria, and the fungal phyla were Ascomycota, Basidiomycota, and Mortierellomycota. The major metabolic pathways of bacteria and fungi in different habitats include biosynthesis, degradation/utilisation/assimilation and generation of precursor metabolites and energy. The bacterial phyla were significantly correlated with soil moisture, sucrase, catalase, and urease (P < 0.05), whereas the fungal phyla were significantly correlated with soil catalase (P < 0.05). In addition, the morphological characteristics of M. ruthenica are significantly correlated with soil moisture, catalase, phosphatase, available potassium, Actinobacteria, Myxococcota, and Patescibacteria. These findings indicate that soil microorganisms, soil physical and chemical properties, and enzyme activities in different habitats are related to structural characteristics, which in turn can affect the morphology of M. ruthenica.
本研究旨在探讨黄花苜蓿的形态特征与微生物群落结构以及土壤环境因子之间的相关性。从黄花苜蓿以及典型草原（C1）、荒漠化草原（C2）和湿地草甸（C3）下的根际土壤和非根际土壤中采集土壤样本。黄花苜蓿的形态特征、土壤理化性质和酶活性在不同生境间存在显著差异（P < 0.05）。枝条长度和茎直径在C2样地中最大，叶面积和叶体积在C3样地中最大。土壤有机质、碱解氮和速效钾含量以及土壤蔗糖酶和土壤磷酸酶活性的排序为C3 > C1 > C2。微生物群落组成也因生境而异。优势细菌门类为变形菌门、放线菌门和酸杆菌门，真菌门类为子囊菌门、担子菌门和被孢霉门。不同生境中细菌和真菌的主要代谢途径包括生物合成、降解/利用/同化以及前体代谢物和能量的产生。细菌门类与土壤湿度、蔗糖酶、过氧化氢酶和脲酶显著相关（P < 0.05），而真菌门类与土壤过氧化氢酶显著相关（P < 0.05）。此外，黄花苜蓿的形态特征与土壤湿度、过氧化氢酶、磷酸酶、速效钾、放线菌门、粘球菌门和微枝菌门显著相关。这些研究结果表明，不同生境中的土壤微生物、土壤理化性质和酶活性与结构特征相关，进而会影响黄花苜蓿的形态。
