Speed Forest, Teel Alec, Futia Gregory L, Restrepo Diego, Gibson Emily A
Department of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
bioRxiv. 2025 Jul 30:2025.07.24.666645. doi: 10.1101/2025.07.24.666645.
We utilize periodic structured illumination with pseudo-HiLo (pHiLo) image reconstruction for in vivo voltage imaging. We demonstrate reduced signal from out-of-focus cells, that contaminates voltage activity for in-focus cells of interest, with pHiLo compared to traditional widefield recordings taken with uniform illumination and pseudo-widefield (pWF) reconstructions. We discuss tradeoffs between signal-to-background ratio, signal-to-noise ratio and temporal resolution for pHiLo in the context of high-speed voltage imaging in awake mice.
我们利用具有伪高低频（pHiLo）图像重建的周期性结构光照进行体内电压成像。与采用均匀光照和伪宽场（pWF）重建的传统宽场记录相比，我们证明了使用pHiLo时，来自离焦细胞的信号减少，而这些信号会干扰感兴趣的聚焦细胞的电压活动。我们在清醒小鼠的高速电压成像背景下讨论了pHiLo在信号背景比、信噪比和时间分辨率之间的权衡。
