Xiang Xin, Guo Yujiao, Zhao Liran, Gao Wei, Li Mao, Zhao Pengju, Wang Zhengguang
College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Hainan Institute, Zhejiang University, Yongyou Industry Park, Yazhou Bay Sci-Tech City, Sanya, 572000, China.
Sci Data. 2025 Aug 6;12(1):1373. doi: 10.1038/s41597-025-05734-x.
Hu sheep is an indigenous breed in China, renowned for its prolificacy. However, Hu sheep have smaller statures compared to other meat sheep breeds, necessitating improvement. Therefore, further research is required to explore the underlying molecular genetic mechanisms of body size traits in Hu sheep. In this study, whole genome sequencing was conducted on 300 Hu sheep with an average depth of 16.51X. A total of 9.53 T of high-quality sequencing data was generated. After quality controlled, Q30 range of clean reads was 86.76% to 95.46%, and GC range was 41.52% to 44.48%. Subsequently, we identified 23274312 single nucleotide polymorphisms (SNPs) and 64759 structural variations (SVs) through a series of bioinformatics analyses. Genome-wide association studies (GWAS), including SNP-GWAS and SV-GWAS, were performed in combination with five body size traits. Furthermore, domestication adaptation regions within the Hu sheep population were explored through selection signatures analysis. This dataset provides a valuable genetic resource for sheep breeding, and serves as a reference for the application of SVs in sheep economic traits.
湖羊是中国的本土品种，以其多产而闻名。然而，与其他肉羊品种相比，湖羊的体型较小，需要进行改良。因此，需要进一步研究来探索湖羊体型性状的潜在分子遗传机制。在本研究中，对300只湖羊进行了全基因组测序，平均深度为16.51X。共产生了9.53 T的高质量测序数据。经过质量控制后，clean reads的Q30范围为86.76%至95.46%，GC范围为41.52%至44.48%。随后，通过一系列生物信息学分析，我们鉴定出23274312个单核苷酸多态性（SNP）和64759个结构变异（SV）。结合五个体型性状进行了全基因组关联研究（GWAS），包括SNP-GWAS和SV-GWAS。此外，通过选择信号分析探索了湖羊群体内的驯化适应区域。该数据集为绵羊育种提供了宝贵的遗传资源，并为SVs在绵羊经济性状中的应用提供了参考。
