College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin, China.
Anim Biotechnol. 2023 Nov;34(4):1214-1222. doi: 10.1080/10495398.2021.2016432. Epub 2021 Dec 20.
The growth traits are economically important traits in sheep. Improving growth rates will increase the profitability of producers. The aim of this study was to identify alleles of (encoding T-cell surface glycoprotein CD8 beta chain) that are aberrantly expressed in different tissues and to assess the effects and associations of its different genotypes on weight and size traits in sheep. Using quantitative real-time reverse transcription PCR arrays, expression profiling of was performed in various organs and tissues. was ubiquitously expressed, with very high expression in the lung, spleen, lymph, duodenum, and liver. One intronic mutation (chr3:62,718,030 (Oar_rambouillet_v1.0, same below) G > A) was identified using pooled DNA sequencing. Subsequently, the variants (AA, AG, and GG) were genotyped using the KASPar PCR single nucleotide polymorphism (SNP) genotyping system. The results of association analysis with body weight and body size traits in 1304 sheep showed that increases in multiple phenotypic traits correlated with the AA genotype (body weight, < 0.05; body length, < 0.05). Thus, SNP chr3:62,718,030 G > A is a promising molecular marker for marker-assisted selection in sheep breeding.
生长性状是绵羊经济重要的性状。提高生长速度将提高生产者的盈利能力。本研究旨在鉴定在不同组织中异常表达的 （编码 T 细胞表面糖蛋白 CD8β链）的等位基因，并评估其不同基因型对绵羊体重和体型性状的影响和关联。使用定量实时逆转录 PCR 阵列，在各种器官和组织中进行了 的表达谱分析。 在肺、脾、淋巴、十二指肠和肝脏中表达水平非常高，在所有组织中均有表达。通过对混合 DNA 测序，发现了一个内含子突变（chr3:62,718,030（Oar_rambouillet_v1.0，下同）G > A）。随后，使用 KASPar PCR 单核苷酸多态性（SNP）基因分型系统对变体（AA、AG 和 GG）进行了基因分型。在 1304 只绵羊的体重和体型性状的关联分析结果表明，多个表型性状的增加与 AA 基因型相关（体重， < 0.05；体长， < 0.05）。因此，SNP chr3:62,718,030 G > A 是绵羊育种中标记辅助选择的有前途的分子标记。
