sp. nov., an actinomycete isolated from a phosphate mine.

Two Gram-staining-positive, non-motile and non-spore-forming bacterial strains, designated as 9H2 and YDN13, were isolated from a phosphate mine in Yunnan Province, China. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains 9H2 and YDN13 belong to the genus . Results show that strains 9H2 and YDN13 occupied a distinct position within the genus and showed the highest 16S rRNA gene sequence similarity of 97.6% with the type strains of CCTCC AB 2020140, followed by 97.5% with DSM 16089 and 97.3% with CCTCC AA 2018028. In the phylogenetic trees based on 16S rRNA gene sequences, strains 9H2 and YDN13 were closely related to CCTCC AA 2018028 and YM 10-847. Strains 9H2 and YDN13 showed the highest values of an orthologous average nucleotide identity (84%) and a digital DNA-DNA hybridization (49%) with CCTCC AA 2018028, followed by an orthologous average nucleotide identity of 76.0% and a digital DNA-DNA hybridization value of 17.9% with CCTCC AB 2020140. The respiratory menaquinones were MK-11, MK-12, MK-10 and MK-13. The major fatty acid profile contained anteiso-C, iso-C and anteiso-C. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids and one unidentified glycolipid. The whole-cell sugars included galactose and glucose. The peptidoglycan hydrolysate contained glutamate, glycine, ornithine and alanine. The DNA G+C contents were 71.1 mol%. Phenotypic, phylogenetic, chemotaxonomic and genomic analyses revealed that strains 9H2 and YDN13 represent a novel species of , for which the name sp. nov. was proposed. The type strain is 9H2 (MCCC 1K08680=KCTC 59053=CGMCC 1.60061).