Ma Huan, Zhen Jing, Feng Fei, Quan Shujing, Hu Hong, Lei Gao, Diao Wentao, Liu Dehai, Li Shanshan, Li Lei, Wang Xueyan, Xie Fuhong
Henan Engineering Research Center of Industrial Enzymes, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
Henan Key Laboratory of Microbial Engineering, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
Int J Syst Evol Microbiol. 2025 Aug;75(8). doi: 10.1099/ijsem.0.006849.
Two Gram-staining-positive, non-motile and non-spore-forming bacterial strains, designated as 9H2 and YDN13, were isolated from a phosphate mine in Yunnan Province, China. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains 9H2 and YDN13 belong to the genus . Results show that strains 9H2 and YDN13 occupied a distinct position within the genus and showed the highest 16S rRNA gene sequence similarity of 97.6% with the type strains of CCTCC AB 2020140, followed by 97.5% with DSM 16089 and 97.3% with CCTCC AA 2018028. In the phylogenetic trees based on 16S rRNA gene sequences, strains 9H2 and YDN13 were closely related to CCTCC AA 2018028 and YM 10-847. Strains 9H2 and YDN13 showed the highest values of an orthologous average nucleotide identity (84%) and a digital DNA-DNA hybridization (49%) with CCTCC AA 2018028, followed by an orthologous average nucleotide identity of 76.0% and a digital DNA-DNA hybridization value of 17.9% with CCTCC AB 2020140. The respiratory menaquinones were MK-11, MK-12, MK-10 and MK-13. The major fatty acid profile contained anteiso-C, iso-C and anteiso-C. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids and one unidentified glycolipid. The whole-cell sugars included galactose and glucose. The peptidoglycan hydrolysate contained glutamate, glycine, ornithine and alanine. The DNA G+C contents were 71.1 mol%. Phenotypic, phylogenetic, chemotaxonomic and genomic analyses revealed that strains 9H2 and YDN13 represent a novel species of , for which the name sp. nov. was proposed. The type strain is 9H2 (MCCC 1K08680=KCTC 59053=CGMCC 1.60061).
从中国云南省的一个磷矿中分离出两株革兰氏染色阳性、不运动且不产芽孢的细菌菌株，分别命名为9H2和YDN13。基于16S rRNA基因序列的系统发育分析表明，菌株9H2和YDN13属于[具体属名未给出]属。结果显示，菌株9H2和YDN13在[具体属名未给出]属内占据独特位置，与[具体菌株名未给出]CCTCC AB 2020140的模式菌株的16S rRNA基因序列相似性最高，为97.6%，其次与[具体菌株名未给出]DSM 16089的相似性为97.5%，与[具体菌株名未给出]CCTCC AA 2018028的相似性为97.3%。在基于16S rRNA基因序列的系统发育树中，菌株9H2和YDN13与[具体菌株名未给出]CCTCC AA 2018028和[具体菌株名未给出]YM 10 - 847密切相关。菌株9H2和YDN13与[具体菌株名未给出]CCTCC AA 2018028的直系同源平均核苷酸同一性最高值为84%，数字DNA - DNA杂交值为49%，其次与[具体菌株名未给出]CCTCC AB
