Periquet B, Bailly A, Periquet A, Ghisolfi J, Thouvenot J P
Int J Vitam Nutr Res. 1985;55(3):245-51.
Rats were fed vitamin A deficient diets (-A) or supplemented with vitamin A (+A) (4.4 mg retinol equivalents/kg diet), either without (-RA) or with retinoic acid (+RA) (12 mg/kg diet) supplementation for up to six weeks. Plasma and liver levels as well as the subcellular localization of vitamin A were determined. In rats reared on the vitamin A rich diet the localization of retinyl palmitate (principal reserve form) is shown to be dependent on age. Two pools exist, i.e. one consisting of the nuclear and mitochondrial-lysosomal fractions and the other containing the microsomal and cytosol fractions. A rapid replenishment of mitochondrial-lysosomal fractions occurs in the first weeks after the weaning. During six weeks of deficient diet an identical mobilization was seen from the different subcellular fractions. Supplementation with RA caused an immediate and sustained reduction of serum vitamin A levels but did not disturb the subcellular localization of retinyl palmitate. A relationship between these phenomena and the subcellular distribution of the retinyl palmitate hydrolase (RPH) and the cellular vitamin A binding proteins (CRBP) is likely to exist.
将大鼠分为两组，分别喂食缺乏维生素A的日粮（-A）或补充了维生素A的日粮（+A）（4.4毫克视黄醇当量/千克日粮），且两组均再分为不补充视黄酸（-RA）或补充视黄酸（+RA）（12毫克/千克日粮）两组，持续喂养六周。测定血浆和肝脏中的维生素A水平以及维生素A的亚细胞定位。结果表明，在富含维生素A的日粮中饲养的大鼠，棕榈酸视黄酯（主要储备形式）的定位取决于年龄。存在两个库，一个由细胞核和线粒体-溶酶体部分组成，另一个包含微粒体和细胞质部分。断奶后的头几周，线粒体-溶酶体部分会迅速补充。在六周的缺乏日粮喂养期间，不同亚细胞部分出现了相同的动员情况。补充视黄酸导致血清维生素A水平立即且持续降低，但并未干扰棕榈酸视黄酯的亚细胞定位。这些现象与棕榈酸视黄酯水解酶（RPH）和细胞维生素A结合蛋白（CRBP）的亚细胞分布之间可能存在关联。
