用于局灶性细菌感染病因诊断的血浆数字滴度聚合酶链反应
Plasma ddPCR for etiological diagnosis of focal bacterial infections.
作者信息
Zhang Yongfeng, Weng ShanShan, Ma Xueqing, Lin Ling, Chen Yueliang, Feng Binbin, Xia Jiang, Ye Jiayuan, Zhou Hua, Yu Yunsong, Zhao Dongdong
机构信息
Department of Infectious Diseases, Jiangshan People's Hospital, Quzhou, China.
Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
出版信息
Front Med (Lausanne). 2025 Jul 24;12:1613077. doi: 10.3389/fmed.2025.1613077. eCollection 2025.
BACKGROUND
Plasma droplet digital polymerase chain reaction (ddPCR) has been used for pathogen detection and has shown good diagnostic value, but no studies have yet demonstrated its application in focal infections. Herein a pilot study using plasma ddPCR to diagnose focal infection is discussed.
METHODS
Eight patients with a diagnosed focal infection who underwent plasma ddPCR for bacterial detection between 2021 and 2022 in Sir Run Run Shaw hospital were enrolled in this retrospective single-center pilot study. Results of ddPCR and focal cultures were compared as well as the turnaround time of two methods and other clinical data.
RESULTS
In 7/8 cases, plasma ddPCR results were consistent with focal culture results. The turnaround time for ddPCR was 2.5 h, significantly lower than the average time for focal cultures of 2.63 days.
CONCLUSION
This pilot study indicates the promising value of the plasma ddPCR method in the rapid diagnosis of focal infection. If larger studies support the findings here, this method can help improve diagnostic accuracy and guide treatment decisions in suspected focal infections.
背景
血浆液滴数字聚合酶链反应（ddPCR）已用于病原体检测并显示出良好的诊断价值，但尚无研究证明其在局灶性感染中的应用。本文讨论了一项使用血浆ddPCR诊断局灶性感染的初步研究。
方法
本回顾性单中心初步研究纳入了2021年至2022年期间在邵逸夫医院接受血浆ddPCR细菌检测的8例确诊局灶性感染患者。比较了ddPCR结果与局灶性培养结果，以及两种方法的周转时间和其他临床数据。
结果
在7/8的病例中，血浆ddPCR结果与局灶性培养结果一致。ddPCR的周转时间为2.5小时，显著低于局灶性培养的平均时间2.63天。
结论
这项初步研究表明血浆ddPCR方法在局灶性感染的快速诊断中具有潜在价值。如果更大规模的研究支持此处的发现，该方法可有助于提高诊断准确性，并指导疑似局灶性感染的治疗决策。
Zotero 插件