Nehru Banoth Sai, Vasu Jayalakshmi, Vivek Srinivas Mouttou, Muthaiah Muthuraj, Mukhopadhyay Hirak Kumar
Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, 605 009, India.
Government Hospital for Chest Diseases, Puducherry, 605 006, India.
Curr Microbiol. 2025 Aug 8;82(9):442. doi: 10.1007/s00284-025-04416-6.
E. coli is one of the first commensal bacteria to colonize the chicken gut. It may act as a source for the spread of antibiotic resistance to human via the food chain and contamination of the environment. Isolation and characterization of such E. coli from commercial broilers in retail outlets of Southern India were carried out. Eighty-three E. coli isolates (76.9%) were obtained from cloacal/meat swabs (108 samples). Phenotypically, 78.3% of isolates were ESBL producers, 69.9% were fluoroquinolone-resistant, and 6% were carbapenemase producers. Genotypically, the blaSHV, blaTEM, and blaCTX-M were present in 48.2%, 43.4%, and 10.8% of the isolates, respectively. These isolates also carried fluoroquinolone-resistant genes viz qnrB (31.3%) and qnrS (34.9%) but not carbapenemase genes. Overall, ESBL were identified in 72.3% of isolates and fluoroquinolone-resistance genes in 51.8%. Strikingly, 53% of the isolates were multidrug-resistant, with both ESBL and fluoroquinolone-resistant genes. The study revealed the presence of MDR E. coli strains in broiler meat at retail outlets indicating the potential public health risks.
大肠杆菌是最早在鸡肠道定殖的共生细菌之一。它可能通过食物链和环境污染成为抗生素耐药性传播给人类的源头。对印度南部零售网点商业肉鸡中的此类大肠杆菌进行了分离和鉴定。从泄殖腔/肉拭子（108份样本）中获得了83株大肠杆菌分离株（76.9%）。表型上，78.3%的分离株产超广谱β-内酰胺酶（ESBL），69.9%对氟喹诺酮耐药，6%产碳青霉烯酶。基因型上，blaSHV、blaTEM和blaCTX-M分别存在于48.2%、43.4%和10.8%的分离株中。这些分离株还携带氟喹诺酮耐药基因qnrB（31.3%）和qnrS（34.9%），但不携带碳青霉烯酶基因。总体而言，72.3%的分离株鉴定出ESBL，51.8%鉴定出氟喹诺酮耐药基因。引人注目的是，53%的分离株对多种药物耐药，同时携带ESBL和氟喹诺酮耐药基因。该研究揭示了零售网点肉鸡中存在多重耐药大肠杆菌菌株，表明存在潜在的公共卫生风险。
